# US 8,570,495 B2 · Whole blood immunity measuring device and whole blood immunity measuring method

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- Number: US 8,570,495 B2
- Document ID: US8570495B2
- Status: Granted
- Kind: B2
- Application number: 13/237,662
- Filed: 2011-09-20
- Published: 2012-03-29
- Granted: 2013-10-29
- Earliest claimed: 2010-09-24
- Assignees: Horiba, Ltd.
- Applicants: Takaaki Shindo; Itsuo Ito
- Inventors: Takaaki Shindo; Itsuo Ito
- Web page: https://patentgrep.com/patent/US8570495B2

## CPC

- G01N 33/726: {Devices}
- G01N 21/5907: {Densitometers}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A whole blood immunity measuring device includes a hemolytic reagent supply device that supplies a hemolytic reagent to a whole blood sample, a first light source that irradiates light on a first blood sample as being the whole blood sample to which the hemolytic reagent is added, a first light detection device that detects transmitted first light intensity, and a Hgb calculation part that calculates concentration of hemoglobin based on the first light intensity. An immunoreagent supply device supplies an immunoreagent to the first sample, a whole second light source irradiates light on a second blood sample as being the first sample to which the immunoreagent is added. A second light detection device detects transmitted second light intensity, and a measuring object calculation part that calculates a concentration of the measuring object based on the second light intensity.

## Claims

### Claim 1

A whole blood immunity measuring device comprising
a hemolytic reagent supply device configured to supply a hemolytic reagent to a whole blood sample,
a first light source configured to irradiate light on a first sample as being the whole blood sample to which the hemolytic reagent is added,
a first light detection device configured to receive the light emitted from the first light source and transmitting through the first sample and to detect first light intensity as being intensity of the received light,
a Hemoglobin (Hgb) calculation part configured to calculate a hemoglobin concentration in the whole blood sample based on the first light intensity detected by the first light detection device,
an immunoreagent supply device configured to supply an immunoreagent to the first sample,
a second light source configured to irradiate light on a second sample as being the first sample to which the immunoreagent is added,
a second light detection device configured to receive the light emitted from the second light source and transmitting through the second sample and to detect second light intensity as being intensity of the received light, and
a measuring object calculation part configured to calculate a concentration of the measuring object based on the second light intensity in the process of an immune reaction of the measuring object to the immune component in the immunoreagent after the immunoreagent is added by the immunoreagent supply device and the concentration of hemoglobin calculated by the Hgb calculation part.

### Claim 2 (depends on 1)

The whole blood immunity measuring device described in claim 1, further comprising
a first cell, a second cell arranged separately from the first cell and a transport device configured to transport the first sample between the first cell and the second cell, wherein
the first light source irradiates the light on the first sample transported to the second cell from the first cell by the transport device,
the immunoreagent supply device supplies the immunoreagent to the first sample returned to the first cell from the second cell by the transport device, and
the second light source irradiates the light on the second sample housed in the first cell.

### Claim 3 (depends on 2)

The whole blood immunity measuring device described in claim 2, wherein
an internal flow channel formed inside of the second cell is in a shape of getting thicker smoothly and gradually from an introducing port from which the first sample is introduced and a lead-out port from which the sample is lead out headed to a center part viewed from an optical axis direction of the light from the first light source.

### Claim 4 (depends on 3)

The whole blood immunity measuring device described in claim 3, wherein
a width in a direction orthogonal both to the direction of the flow of the sample and to a direction of an optical axis of the light from the first light source in the center part of the internal flow channel of the second cell is 1.2˜2.0 times as long as a width in the orthogonal direction in the introducing port and the lead-out port.

### Claim 5 (depends on 1)

The whole blood immunity measuring device described in claim 1, wherein
the measuring object is C-reactive protein.

### Claim 6

A whole blood immunity measuring method comprising;
preparing a first sample by adding a hemolytic reagent to whole blood,
irradiating light on the first sample and measuring first light intensity as being intensity of transmitting through the first sample,
calculating a concentration of hemoglobin in the whole blood based on the first light intensity,
adding the immunoreagent to the first sample and causing an immune reaction of a measuring object in the first sample to an immune component in the immunoreagent,
irradiating light on a second sample as being the first sample to which the immunoreagent is added and measuring the second light intensity as being the intensity of the light transmitting through the second sample in the process of an immune reaction of the measuring object to the immune component, and
calculating a concentration of the measuring object based on the second light intensity and the hemoglobin concentration.