# US 8,570,370 B2 · Compact automated cell counter

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- Number: US 8,570,370 B2
- Document ID: US8570370B2
- Status: Granted
- Kind: B2
- Application number: 12/869,979
- Filed: 2010-08-27
- Published: 2011-09-01
- Granted: 2013-10-29
- Earliest claimed: 2009-08-31
- Assignees: Bio-Rad Laboratories, Inc.
- Applicants: Tom McCollum; Paul Patt; Frank Shen; Daniel Y. Chu; Don Flory; Mike Griffin; Xin Heng; Eli Hefner
- Inventors: Tom McCollum; Paul Patt; Frank Shen; Daniel Y. Chu; Don Flory; Mike Griffin; Xin Heng; Eli Hefner
- Web page: https://patentgrep.com/patent/US8570370B2

## CPC

- C12Q 1/06: Quantitative determination
- G01N 15/1434: Optical arrangements
- G02B 21/0008: {Microscopes having a simple construction, e.g. portable microscopes}
- G02B 21/34: Microscope slides, e.g. mounting specimens on microscope slides
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- G02B: OPTICAL ELEMENTS, SYSTEMS OR APPARATUS

## Abstract

Biological cells in a liquid suspension are counted in an automated cell counter that focuses an image of the suspension on a digital imaging sensor that contains at least 4,000,000 pixels each having an area of 2×2 μm or less and that images a field of view of at least 3 mm2. The sensor enables the counter to compress the optical components into an optical path of less than 20 cm in height when arranged vertically with no changes in direction of the optical path as a whole, and the entire instrument has a footprint of less than 300 cm2. Activation of the light source, automated focusing of the sensor image, and digital cell counting are all initiated by the simple insertion of the sample holder into the instrument. The suspension is placed in a sample chamber in the form of a slide that is shaped to ensure proper orientation of the slide in the cell counter.

## Claims

### Claim 1

An automated cell counter comprising optical components, a sample mount for receiving a sample slide, and a housing retaining said optical components and sample mount, said optical components comprising a light source, a digital imaging sensor, and lenses positioned to direct light from said light source to said digital imaging sensor through said sample mount, said optical components arranged in a linear optical path and arranged such that when a sample slide containing a cell suspension is mounted in said sample mount, an image of said cell suspension is projected onto said digital imaging sensor, said digital imaging sensor comprising at least about 4,000,000 pixels and imaging a field of view at said sample mount of at least 3 mm2, each pixel being 2×2 mm or less in area, wherein the automated cell counter comprises an aspect for correcting curvature of a focus field, and wherein said optical path is vertically oriented and said housing is mounted to a support base that is at least as wide as said housing, said housing and support base each occupying lateral areas of not more than 300 cm2, said optical path having a total height of not more than 20 cm, said housing is 30 cm or less in height and said sample mount is at least 6 cm above said surface.

### Claim 2 (depends on 1)

The automated cell counter of claim 1 wherein said digital sensor contains from about 4,000,000 to about 10,000,000 pixels, each pixel is from about 0.5×0.5 mm to about 2′2 mm in area, and said imaging field is from about 3 mm2 to about 10 mm.

### Claim 3 (depends on 1)

The automated cell counter of claim 1 wherein said digital imaging sensor comprises means for generating contrast values between adjacent regions of said image, said sample mount is of adjustable height along to said optical path, and said cell counter further comprises means for automated adjustment of said height in response to said contrast values until said image is focused.

### Claim 4 (depends on 3)

The automated cell counter of claim 3 further comprising a digital imager that counts cells in said field of view.

### Claim 5 (depends on 4)

The automated cell counter of claim 4 wherein said automated adjustment of said height of said sample mount, and digital imager, are initiated automatically upon insertion of a sample slide in said sample mount.

### Claim 6 (depends on 1)

The automated cell counter of claim 1 wherein said digital imaging sensor is flat, said lenses comprise an achromat lens pair having a focus field at said digital imaging sensor, and said automated cell counter further comprises means for correcting curvature of said focus field.

### Claim 7 (depends on 6)

The automated cell counter of claim 6 wherein said means for correcting curvature of said focus field is a negative lens adjacent to said digital imaging sensor.

### Claim 8 (depends on 1)

The automated cell counter of claim 1 wherein said light source is spectrally limited to produce an image in said imaging sensor that differentiates between live cells and dead cells.

### Claim 9 (depends on 1)

The automated cell counter of claim 1 wherein said digital imaging sensor is a color digital sensor.

### Claim 10 (depends on 1)

The automated cell counter of claim 1 further comprising a quality control slide dimensioned to be mounted on said sample mount and having images printed thereon to simulate live cells and dead cells.

### Claim 11 (depends on 1)

A method for counting cells in a cell suspension, said method comprising:
(a) placing an aliquot of said cell suspension in an internal chamber of a sample slide to form a film of said suspension in said chamber,
(b) creating a digital image of a field of view of at least 3 mm2 within said film on the automated cell counter of claim 1, and
(c) digitally counting said cells in said digital image.

### Claim 12 (depends on 11)

The method of claim 11 wherein said digital sensor contains from about 4,000,000 to about 10,000,000 pixels, each pixel is from about 0.5×0.5 mm to about 2′2 mm in area, and said imaging field is from about 3 mm2 to about 10 mm2.

### Claim 13 (depends on 11)

The method of claim 11 wherein step (b) is performed by a plurality of functions comprising automatic focusing of said digital image by moving said sample mount, detecting whether a vital stain is present in said aliquot, creating digital images of a plurality of focal planes within said internal chamber of said sample slide when a vital stain is detected, and processing images formed on said digital imaging sensor to determine a cell count in said aliquot, are all performed internally within said housing.

### Claim 14 (depends on 11)

The method of claim 11 further comprising (a′) automatically generating contrast values between adjacent regions of said image, and automatically adjusting said sample mount to a height along said optical path in response to said contrast values to focus said image.

### Claim 15 (depends on 11)

The method of claim 11 wherein steps (a′) and (b) are initiated automatically upon insertion of said sample slide in said sample mount.

### Claim 16 (depends on 11)

The method of claim 11 wherein said digital imaging sensor is flat, and said lenses comprise an achromat lens pair having a focus field at said digital imaging sensor and a negative lens positioned to correct curvature of said focus field.

### Claim 17 (depends on 11)

The method of claim 11 wherein said light source is spectrally limited to produce an image in said imaging sensor that differentiates between live cells and dead cells.

### Claim 18 (depends on 11)

The method of claim 11 further comprising treating said cell suspension with a vital stain and thereby producing an image in said imaging sensor that differentiates between live cells and dead cells.

### Claim 19 (depends on 11)

The method of claim 11 wherein said digital imaging sensor is a color digital sensor.

### Claim 20 (depends on 11)

The method of claim 11 wherein step (c) comprises focusing said optical components on a plurality of planes within said internal chamber.