# US 8,569,587 B2 · Soybean cultivar S100312

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- Number: US 8,569,587 B2
- Document ID: US8569587B2
- Status: Granted
- Kind: B2
- Application number: 13/228,223
- Filed: 2011-09-08
- Published: 2013-03-14
- Granted: 2013-10-29
- Earliest claimed: 2011-09-08
- Assignees: M.S. Technologies, L.L.C.
- Applicants: Justin T. Mason
- Inventors: Justin T. Mason
- Web page: https://patentgrep.com/patent/US8569587B2

## CPC

- A01H 6/542: {Glycine max [soybean]}
- A01H 5/10: Seeds
- A23D 9/00: Other edible oils or fats, e.g. shortenings or cooking oils (preservation of other edible oils A23B20/30)
- A23L 11/03: {Soya beans, e.g. full-fat soya bean flakes or grits}
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A23D: EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS (preservation thereof A23B20/00; production, refinement or preservation of animal or vegetable fats or oils C11B, C11C)
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)

## Abstract

A soybean cultivar designated S100312 is disclosed. The invention relates to the seeds of soybean cultivar S100312, to the plants of soybean cultivar S100312, to the plant parts of soybean cultivar S100312, and to methods for producing progeny of soybean cultivar S100312. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar S100312. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar S100312, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar S100312 with another soybean cultivar.

## Claims

### Claim 1

A seed of soybean cultivar S100312, wherein a representative of sample seed of said cultivar is deposited under ATCC Accession No PTA-12375.

### Claim 2 (depends on 1)

A soybean plant, or a part thereof, produced by growing the seed of claim 1.

### Claim 3 (depends on 2)

A tissue culture produced from protoplasts or cells from the plant of claim 2, wherein said cells or protoplasts are produced from a plant part selected from the group consisting of leaf, pollen, ovule, embryo, cotyledon, hypocotyl, meristematic cell, root, root tip, pistil, anther, flower, seed, shoot, stem, pod and petiole.

### Claim 4 (depends on 3)

A soybean plant regenerated from the tissue culture of claim 3.

### Claim 5 (depends on 2)

A method for producing a soybean seed, comprising crossing two soybean plants and harvesting the resultant soybean seed, wherein at least one soybean plant is the soybean plant of claim 2.

### Claim 6 (depends on 5)

A soybean seed produced by the method of claim 5.

### Claim 7 (depends on 6)

A soybean plant, or a part thereof, produced by growing said seed of claim 6.

### Claim 8 (depends on 5)

The method of claim 5, wherein at least one of said soybean plants is transgenic.

### Claim 9 (depends on 2)

A method of producing an herbicide resistant soybean plant, wherein said method comprises introducing a gene conferring herbicide resistance into the plant of claim 2.

### Claim 10 (depends on 9)

A herbicide resistant soybean plant produced by the method of claim 9, wherein the gene confers resistance to a herbicide selected from the group consisting of glyphosate, sulfonylurea, imidazolinone, dicamba, glufosinate, phenoxy proprionic acid, L-phosphinothricin, cyclohexone, cyclohexanedione, triazine, 2,4-Dichlorophenoxyacetic acid, hydroxyphenyl-pyruvate dioxygenase (HPPD) inhibitors and benzonitrile.

### Claim 11 (depends on 2)

A method of producing a pest or insect resistant soybean plant, wherein said method comprises introducing a gene conferring pest or insect resistance into the soybean plant of claim 2.

### Claim 12 (depends on 11)

A pest or insect resistant soybean plant produced by the method of claim 11.

### Claim 13 (depends on 12)

The soybean plant of claim 12, wherein the gene encodes a Bacillus thuringiensis (Bt) endotoxin.

### Claim 14 (depends on 2)

A method of producing a disease resistant soybean plant, wherein said method comprises introducing a gene which confers disease resistance into the soybean plant of claim 2.

### Claim 15 (depends on 14)

A disease resistant soybean plant produced by the method of claim 14.

### Claim 16 (depends on 2)

A method of producing a soybean plant with modified fatty acid metabolism or modified carbohydrate metabolism, wherein the method comprises introducing a gene encoding a protein selected from the group consisting of phytase, fructosyltransferase, levansucrase, α-amylase, invertase, starch branching enzyme and DNA encoding an antisense of stearyl-ACP desaturase into the soybean plant of claim 2.

### Claim 17 (depends on 16)

A soybean plant having modified fatty acid metabolism or modified carbohydrate metabolism produced by the method of claim 16.

### Claim 18

A method of introducing a desired trait into soybean cultivar S100312, wherein the method comprises:
(a) crossing a S100312 plant, wherein a representative sample of seed is deposited under ATCC Accession No PTA-12375, with a plant of another soybean cultivar that comprises a desired trait, wherein said desired trait is selected from the group consisting of male sterility, herbicide resistance, insect resistance, abiotic stress tolerance, modified fatty acid metabolism, modified carbohydrate metabolism, modified seed yield, modified oil percent, modified protein percent, modified lodging resistance, modified shattering, modified iron-deficiency chlorosis and resistance to bacterial disease, fungal disease or viral disease;
(b) selecting one or more progeny plants that have the desired trait;
(c) backcrossing the selected progeny plants with soybean cultivar S100312;
(d) selecting for backcross progeny plants that have the desired trait; and
(e) repeating steps (c) and (d) two or more times in succession to produce selected third or higher backcross progeny plants that comprise the desired trait.

### Claim 19 (depends on 18)

A soybean plant produced by the method of claim 18, wherein the plant has the desired trait and all of the physiological and morphological characteristics of soybean cultivar S100312 listed in Table 1.

### Claim 20 (depends on 19)

The soybean plant of claim 19, wherein the desired trait is herbicide resistance and the resistance is conferred to an herbicide selected from the group consisting of imidazolinone, dicamba, cyclohexanedione, sulfonylurea, glyphosate, glufosinate, phenoxy proprionic acid, L-phosphinothricin, triazine, 2.4-Dichlorophenoxyacetic acid, hydroxyphenyl-pyruvate dioxygenase (HPPD) inhibitors and benzonitrile.

### Claim 21 (depends on 19)

The soybean plant of claim 19, wherein the desired trait is insect resistance and the insect resistance is conferred by a gene encoding a Bacillus thuringiensis endotoxin.

### Claim 22 (depends on 19)

The soybean plant of claim 19, wherein the desired trait is modified fatty acid metabolism or modified carbohydrate metabolism and said desired trait is conferred by a nucleic acid encoding a protein selected from the group consisting of phytase, fructosyltransferase, levansucrase, α-amylase, invertase, starch branching enzyme and DNA encoding an antisense of stearyl-ACP desaturase.

### Claim 23 (depends on 19)

The soybean plant of claim 19, wherein the desired trait is abiotic stress tolerance and said desired trait modifies tolerance to drought, flooding, salinity, temperature or climate change.

### Claim 24 (depends on 2)

A method of introducing a mutation into the genome of soybean cultivar S100312, said method comprising applying a mutagen to the plant of claim 2, or a part thereof, wherein said mutagen is selected from the group consisting of ethyl methanesulphhonate, gamma-rays, and sodium azide.

### Claim 25 (depends on 24)

A mutagenized soybean plant produced by the method of claim 24.

### Claim 26 (depends on 2)

A method of producing a commodity plant product, comprising obtaining the plant of claim 2, or a part thereof, and producing the commodity plant product from said plant or plant part thereof, wherein said commodity plant product is selected from the group consisting of protein concentrate, protein isolate, soybean hulls, meal, flour and oil.