Method for transformation of grasses
AssigneeThe Samuel Roberts Noble Foundation
InventorsZeng-Yu Wang, Rangaraj Nandakumar
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Abstract
The invention provides methods for transforming grass plants with Agrobacterium. The invention allows creation of transgenic grass plants without the need for callus as a target tissue for transformation, thus providing a rapid method for the production of transgenic grass plants. Transgenic grass plants produced by this method are also provided.
Claims13
A method of transforming a grass plant cell comprising contacting a non-stolon or non-rhizome nodal explant, or an inflorescence explant, from a grass plant with an Agrobacterium comprising a DNA of interest, wherein the explant is contacted with the Agrobacterium without first forming a callus culture of cells from the explant, wherein at least a first cell of the explant is transformed with the DNA of interest;
- wherein the grass plant is switchgrass and wherein the explant is excised from the plant prior to contacting the explant with the Agrobacterium.
Dependencies are inferred from the claim text.