Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
AssigneeNovozymes, Inc.
InventorsSuchindra Maiyuran, Randall Kramer, Paul Harris
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Abstract
The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Claims34
An isolated GH61 polypeptide having cellulolytic enhancing activity, selected from the group consisting of:
- (a) a GH61 polypeptide having at least 95% sequence identity to amino acids 18to 246 of SEQ ID NO:
- 2;
- (b) a GH61 polypeptide encoded by a polynucleotide that hybridizes under very high stringency conditions with (i) nucleotides 52 to 875 of SEQ ID NO:
- 1, (ii) the cDNA sequence contained in the mature polypeptide coding sequence of SEQ ID NO:
- 1, or (iii) the full-length complement of (i) or (ii), wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5X SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2X SSC, 0.2% SDS at 70° C.;
- (c) a GH61 polypeptide encoded by a polynucleotide having at least 99% sequence identity to nucleotides 52 to 875 of SEQ ID NO:
- 1 or the cDNA sequence thereof;
- and
- (d) an isolated fragment of a GH61 polypeptide of (a), (b), or (c) that has cellulolytic enhancing activity.
A DNA construct comprising a nucleic acid encoding the signal peptide comprising or consisting of amino acids 1 to 17 of SEQ ID NO:
- 2operably linked to a heterologous nucleic acid.
An isolated GH61 polypeptide, comprising SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.