BPMV-based viral constructs useful for VIGS and expression of heterologous proteins in legumes
AssigneeIowa State University Research Foundation, Inc.
InventorsJohn H. Hill, Chunquan Zhang, Steve Whitham
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Abstract
The invention provides Bean pod mottle virus (BPMV) vectors useful for expression of heterologous proteins in plants such as soybean. The BPMV vectors are also useful for virus-induced gene silencing. The vectors of the invention include modifications of BPMV RNA1 sequences so that infection with the vectors produces only moderate symptoms. The vectors also comprise novel RNA2 vectors which specifically provide for non-translated VIGS constructs and further which do not require in frame insertion of heterologous sequences to be expressed.
Claims4
A Bean Pod mottle virus (BPMV) helper sequence for BPMV vector inoculation in a recipient plant, comprising a BPMV I-Di1 isolate RNA1 sequence that has been modified so that said inoculation produces enhanced symptoms in a recipient plant as compared to a wild type BPMV I-Di1 RNA1, and less severe symptoms as compared to a wild type BPMV K-Ho1 RNA1, and wherein said BPMV I-Di1 isolate RNA1 sequence encodes a polypeptide comprising a BPMV RNA1 helicase having modifications consisting of modifications to the amino acids at position 359 and 365 of the BPMV RNA1 helicase, wherein said amino acid positions are determined by alignment with SEQ ID NO:2 and wherein said BPMV I-Di1 isolate RNA1 sequence encodes a polypeptide having at least 90% identity to SEQ ID NO:8.
Dependencies are inferred from the claim text.