US 8,569,515 B2 · Directed synthesis of oligophosphoramidate stereoisomers

Abstract

The trivalent phosphorous atom of a compound is reacted with a reagent in such a manner that a stable phosphate mimetic or a specifier is formed. Phosphoramidites with a phosphorous atom containing at least one hydroxyl residue which is provided with a protective group are reacted for this purpose with a free hydroxyl group: In the first synthesis cycle the hydroxyl group is linked to a solid support via a cleavable or non-cleavable linker. In further synthesis cycles the hydroxyl group is created by cleavage of the protective group from the growing oligomer. This results in formation of a phosphorous acid triester which is reacted with azides. By selecting suitable monomers for the synthesis which have a defined stereoconformation compounds of Formula 1 are produced in a stereocontrolled manner.

A process for producing a compound comprising the steps of:

(a) providing a solid support to which is attached a hydroxyalkyl group via a cleavable or non-cleavable linker,
(b) supplying a first compound according to Formula 2:
wherein X is a spacer consisting of 2-16 atoms, whereby the spacer is selected from the group consisting of:
branched or linear alkyl,
linear C4-C14 heteroalkyl with one N atom,
branched or linear C4-C14 alkenyl,
branched or linear C4-C14 alkinyl,
bis-(C1-C3)-alkyl-(C6-C10)-aryl,
bis-(C1-C3)-alky-heteroaryl, with 5-6 membered heteroaryl with 1-3 heteroatoms selected from N, O and S,
5-10 membered cycloalkyl,
5-10 membered heteroalkyl structure with 2 heteroatoms, whereby each heteroatom is independently selected from the group consisting of N, O, and S,
5-10 membered cyclo-heteroalkyl structure with 1-5 heteroatoms, whereby each heteroatom is independently selected from the group consisting of N, O, and S;
in which the atom or atoms of X which are connected to phosphoramidate are sp3 C atoms;
in which R2 in the monomer is a moiety selected from the group consisting of
a hydrogen atom,
a core moiety selected from the group consisting of:
a linear or branched C1-C6 alkyl group,
a linear or branched C2-C6 alkenyl group,
a linear or branched C2-C6 alkinyl group,
a 5-10 membered cycloalkyl group,
a 6-10 membered aryl group,
a 5-10 membered heterocyclic group with 1-5 heteroatoms, whereby each heteroatom is independently selected from N, O, and S;
a substituent attached to the core moiety, whereby the substituent is selected from the group consisting of:
a hydrogen atom,
a halogen atom,
a protected carboxyl group,
a protected formyl group,
a C1-C6 acyl group,
a C6-C10 aroyl group,
a protected hydroxyl group,
a C1-C6 acylamino group,
a protected amino group,
a carboxamido group,
a protected mercapto group,
a cyano group,
a nitro group,
a C1-C6 alkoxy group,
a C1-C6 alkoxycarbonyl group,
a C6-C10 aryloxy group,
a C6-C10 aryloxycarbonyl group,
a protected sulfhydryl group,
a C6-C10 aryl- or C1-C6 alkyl-sulfonyl group,
a protected phosphatyl group,
a guanidyl group,
a primary or secondary carboxyamido group,
a detectable moiety,
a C6-C10 aryl group,
a C2-C6 alkenyl group,
a C2-C6 alkinyl group,
a 5-10 membered heteroaryl group;
in which T2 in the monomer is a tether consisting of a linear, branched or cyclic organic moiety comprising 1-30 C— atoms and 0-5 heteroatoms selected from N, O, and S, and 1-3 subunits selected from a carboxy moiety, an amide moiety and a urea moiety, whereby the internal heteroatoms with the exception of a disulfide bond are separated from each other by a minimum of two carbon atoms;
in which
R3 is selected from a first group of substituents consisting of H, C1-C3-alkyl, or
R3 is selected from a second group of substituents consisting of acetyl, monofluor-acetyl, and difluor-acetyl;
in which R4 is selected from the group consisting of H, C1-C3 alkyl, and C6-C10 aryl;
in which R3 and R4 are optionally covalently connected to form a —(CH2)n-bridge with n=3 or n=4;
in which R5 is selected from the group consisting of H, C1-C3 alkyl, and C6-C10 aryl;
and
in which PG 1 is either a photo-cleavable linker selected from the group consisting of NVOC and NPPOC, or PG 1 is an acid-cleavable linker selected from the group consisting of monomethoxytrityl, dimethoxytrityl, trimethoxytrityl, 9-phenylxanthine-9-yl (Pixyl), and 9-(p-methoxyphenyl)xanthine-9-yl (MOX);
(c) in case that R3 consists of the first group of substituents or in case R3 is covalently linked to R4 performing the step of activating the compound of step (b) with an activator selected from
IH-tetrazole, dicyano-imidazole,
N-phenylimidazolium triflate, N-methylbenzimidazolium triflate, and
N-(cyanomethyl)pyrrolidinium triflate,
or in case R3 consists of the second group of substituents
performing the step of activating the compound of step (b) with N,N,N′,N′-tetramethylguanidine thereby providing an activated compound;
(d) reacting the activated compound of step (c) with the hydroxyl group of step (a), thereby forming a phosphate triester,
whereby in the case of R3 being selected from the first group a beta-amino function with R6=H is formed, and
whereby in the case of R3 being selected from the second group an acylated beta-amino function is formed;
(e) in case that R3 consists of the first group of substituents, performing the step of capping the beta amino group of step (d) with acetanhydride thereby introducing R6=acetyl, and proceeding with step (f),
otherwise directly proceeding with step (f);
(f) reacting the capped compound of step (e) or the compound of step (d) with a compound of Formula 3
in which R1 is selected independently from R2 and R1 is selected from the group consisting of:
a hydrogen atom,
a halogen atom,
a protected carboxyl group,
a protected formyl group,
a C1-C6 acyl group,
a C6-C10 aroyl group,
a protected hydroxyl group,
a C1-C6 acylamino group,
a protected amino group,
a carboxamido group,
a protected mercapto group,
a cyano group,
a nitro group,
a C1-C6 alkoxy group,
a C1-C6 alkoxycarbonyl group,
a C6-C10 aryloxy group,
a C6-C10 aryloxycarbonyl group,
a protected sulfhydryl group,
a C6-C10 aryl- or C1-C6 alkyl-sulfonyl group,
a protected phosphatyl group,
a guanidyl group,
a primary or secondary carboxyamido group,
a detectable moiety,
a C6-C10 aryl group,
a C2-C6 alkenyl group,
a C2-C6 alkinyl group,
a 5-10 membered heteroaryl group,
in which T1 is a further tether and is selected independently from T2, and T1 is selected from the group consisting of a linear, branched or cyclic organic moiety comprising 1-30 C— atoms and 0-5 heteroatoms selected from N, O, and S, and 1-3 subunits selected from a carboxy moiety, an amide moiety and a urea moiety, whereby the internal heteroatoms with the exception of a disulfide bond are separated from each other by a minimum of two carbon atoms,
in which Acc is an electron acceptor selected from the group consisting of:
a methyl-sulfonyl,
a C6-C10 aryl-sulfonyl,
a C5-C6 heteroaryl-sulfonyl with 1 or 2 heteroatoms selected from N, O, and S,
a C5-C10 cycloalkyl-sulfonyl,
a 6 membered electron-deficient aromatic ring, and
heteroaromatic ring with a six-membered heterocycle with at least one alkylated N-atom in ortho- or para-position, said heterocycle being selected from the group consisting of pyridinium, pyrimidinium and chinolinium;
thereby forming a protected and immobilized monomer;
(g) capping the non-reacted free hydroxyl groups of the solid support with acetanhydride or pivaloylanhydride;
(h) alternatively, if PG 1 is a photo-cleavable protective group, the method further includes the step of removing PG 1 by applying UV light to the protected and immobilized monomer, or if PG 1 is an acid-labile protective group, the method further includes the step of removing PG 1 by applying acidic conditions to the protected and immobilized monomer,
whereby on the immobilized monomer a new free hydroxyl group is formed;
(i) providing a further compound according to Formula 2, whereby R2, R3, R4, R5, T2, PG1 and X are selected independently from each other and independently from the respective R2, R3, R4, R5, T2, PG 1 and X in each other monomer;
if R3 is selected from the first group of substituents
or in if R3 is covalently linked to R4;
(j) performing the step of activating the compound of step (b) with an activator selected from
IH-tetrazole, dicyano-imidazole,
N-phenylimidazolium triflate, N-methylbenzimidazolium triflate, and
N-(cyanomethyl)pyrrolidinium triflate,
if R3 is selected from the second group of substituents, the method further includes
activating the compound of step (b) with N,N,N′,N′-tetramethylguanidine, thereby providing an activated further compound;
(k) reacting the free hydroxyl group formed during step (h) with the activated compound of step
(l) thereby forming a phosphate triester,
wherein if R3 is selected from the first group a beta-amino function with R6=H is formed;
and
wherein if R3 is selected from the second group an acylated beta-amino function is formed;
(m) capping the beta amino group of step (k) with acetanhydride in case that R3 consists of the first group of substituents, thereby introducing R6=acetyl, and proceeding with step (l), otherwise directly proceeding with step (m);
(n) reacting the compound of step (e) or the compound of step (d) with a further compound of Formula 3
wherein Acc, R1 and T1 are selected independently from each other and independently from Acc, R1 and T1 of the other monomer(s),
thereby forming a protected and immobilized oligomer with a further monomer;
(o) capping free hydroxyl groups with acetanhydride or pivaloylanhydride;
(p) applying, if PG1 is a photo-cleavable protective group UV light to remove the protected and immobilized monomer, or an acid solution if PG1 is an acid-labile protective group to remove PG1 from the protected and immobilized monomer to from a new free hydroxyl group
(q) repeating steps (i) to (p) z times, whereby z is an integer between 0 and 8;
(r) cleaving the permanent protective groups of the phosphoramidate moieties and the protective groups attached to R1 and R2 under basic conditions or under reducing conditions, if a cleavable linker was used in step (a) the linker is cleaved from the solid support to form a compound according to Formula 1;
wherein B in Formula (1) is H,
if a non-cleavable linker was used in step (a) a compound according to Formula (1a) is formed;
wherein B in Formula (1a) is a non-cleavable linker;
and
wherein A is selected from the group consisting of:
a hydroxyl group,
a phosphate, and
a C3-C10 monoalkyl phosphate (—O—P═O(O)—O-alkyl), wherein said monoalkyl is linear, branched or a cycle and optionally substituted with a moiety selected from the group consisting of a detectable moiety and a reactive group selected from —SH, —NH2, —O—NH2, —NH—NH2, —C(═O)H, —COOH, —CH═CH2, —C═CH, and —OH;
in which B is selected independently from A and is selected from the group consisting of
a hydrogen atom, and
a C3-C10 alkyl, wherein said alkyl is linear, branched or a cycle, and optionally substituted with a moiety selected from the group consisting of a detectable moiety and reactive functional group selected from —SH, —NH2, —O—NH2, —NH—NH2, —C(═O)H, —COOH, —CH═CH2, —C═CH, —OH, and a solid support;
wherein n is an integer denoting the number of monomers, and n is equal to or greater than 2;
wherein each monomer Acc, R1, R2, T1, T2 and X are selected independently from each other and from the Acc, R1, R2, T1, T2 and X moieties of the other monomer(s);
wherein Acc is an electron acceptor selected from the group consisting of:
a methyl-sulfonyl,
a C6-C10 aryl-sulfonyl,
a C5-C6 heteroaryl-sulfonyl with 1 or 2 heteroatoms selected from N, O, and S,
a C5-C10 cycloalkyl-sulfonyl,
a 6 membered electron-deficient aromatic ring, and
a six-membered heterocycle with at least one alkylated N-atom in ortho- or para-position, said heterocycle being selected from the group consisting of pyridinium, pyrimidinium and chinolinium;
in which X is a spacer part consisting of 2-16 atoms, whereby the spacer part is selected from the group consisting of
a branched or linear alkyl,
a linear C4-C14 heteroalkyl with one N atom
a branched or linear C4-C14 alkenyl,
a branched or linear C4-C14 alkinyl,
a bis-(C1-C3)-alkyl-(C6-C10)-aryl,
a bis-(C1-C3)-alkyl-heteroaryl, with 5-6 membered heteroaryl with 1-3 heteroatoms selected from N, O and S
a 5-10 membered cycloalkyl
a 5-10 membered heteroalkyl structure with 2 heteroatoms, whereby each heteroatom is independently selected from N, O, and S, and
a 5-10 membered cyclo-heteroalkyl structure with 1-5 heteroatoms, whereby each heteroatom is independently selected from N, O, and S;
in which the atom or atoms of X which are connected to phosphoramidate or A are sp3 C atoms;
in which R1 is selected independently from R2, and R1 is directly or via a tether T1 attached to Acc;
wherein R2 is selected independently from R1, and R2 is directly or via a tether T2 to a C atom or (if present) N atom of X;
wherein R1 and R2 are selected from the group consisting of
a hydrogen atom,
a core moiety selected from the group consisting of
a linear or branched C1-C6 alkyl group,
a linear or branched C2-C6 alkenyl group,
a linear or branched C2-C6 alkinyl group,
a 5-10 membered cycloalkyl group,
a 6-10 membered aryl group,
a 5-10 membered heterocyclic group with 1-5 heteroatoms, whereby each heteroatom is independently selected from N, O, and S,
a substituent attached to the core moiety, whereby the substituent is selected from the group consisting of
a hydrogen atom,
a halogen atom,
a carboxyl group,
a formyl group,
a C1-C6 acyl group,
a C6-C10 aroyl group,
a hydroxyl group,
a C1-C6 acylamino group,
an amino group,
a carboxamido group,
a C1-C6 alkylmercapto group,
a cyano group,
a nitro group,
a C1-C6 alkoxy group,
a C1-C6 alkoxycarbonyl group,
a C6-C10 aryloxy group,
a C6-C10 aryloxycarbonyl group,
a sulfhydryl group,
a C6-C10 aryl- or C1-C6 alkyl-sulfonyl group,
a phosphatyl group,
a guanidyl group,
a primary or secondary C1-C6 carboxamido group,
a detectable moiety,
a C6-C1 aryl group,
a C2-C6 alkenyl group,
a C2-C6 alkinyl group, and
a 5-10 membered heteroaryl group,
in which the tethers T1 and T2 are selected independently from each other, and a tether consists of a linear, branched or cyclic organic moiety comprising 1-30 C-atoms and 0-5 heteroatoms selected from N, O, and S, and 1-3 subunits selected from a carboxy moiety, an amide moiety and a urea moiety, whereby the internal heteroatoms with the exception of a disulfide bond are separated from each other by a minimum of two carbon atoms;
wherein for each monomer X, R1 and R2, and T1 and T2 are selected independently from the group consisting of X, R1 and R2, and T1 and T2 from other monomers, and
wherein the compound of Formula 1 or Formula 1a is a defined stereoisomer.

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Directed synthesis of oligophosphoramidate stereoisomers

Life of this patent

Directed synthesis of oligophosphoramidate stereoisomers

Life of this patent

Abstract

Claims10