Quaternary nitrogen heterocyclic compounds for detecting aqueous monosaccharides in physiological fluids
AssigneeChris Geddes
InventorsChris D. Geddes, Ramachandram Badugu, Joseph R. Lakowicz
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Abstract
Quaternary nitrogen heterocyclic boronic acid-containing compounds are described, which are sensitive to glucose and fructose, as well as a variety of other physiologically important analytes, such as aqueous chloride and iodide, and a method of using the compounds. Also disclosed is a contact lens doped with the quaternary nitrogen heterocyclic boronic acid-containing compound, and a method of using the doped contact lens to measure the concentration of analyte in tears under physiological conditions.
Claims13
A fluorescence compound selected from the group consisting of:
- wherein X is chloride, bromide or iodide.
A method of measuring the concentration of an analyte in a physiological fluid, said method comprising:
- (a) contacting a fluorescence compound selected from the group consisting of:
- wherein X is chloride, bromide or iodide,
- with the physiological fluid for sufficient time to at least partially interact or react with the analyte;
- and
- (b) measuring the optical signal of the fluorescence compound in the presence of the analyte for a sufficient time to determine the concentration of analyte in the physiological fluid.
An assay kit for measuring the concentration of an analyte in a physiological fluid comprising a container containing a fluorescence compound selected from the group consisting of:
- wherein X is chloride, bromide or iodide.
An ophthalmic sensor comprising:
- a polymer matrix comprising a fluorescence compound contracting at least the outer surface of the polymer matrix, wherein the fluorescence compound interacts or reacts with an analyte to provide an optical signal which is indicative of the analyte concentration in an ocular fluid and wherein the fluorescence compound is at least one member selected from the group consisting of:
- wherein X is chloride, bromide or iodide.
Dependencies are inferred from the claim text.