# US 8,569,458 B2 · Xylose utilizing Zymomonas mobilis with improved ethanol production in biomass hydrolysate medium

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- Number: US 8,569,458 B2
- Document ID: US8569458B2
- Status: Granted
- Kind: B2
- Application number: 13/795,148
- Filed: 2013-03-12
- Published: 2013-07-11
- Granted: 2013-10-29
- Earliest claimed: 2010-12-17
- Assignees: E. I. Du Pont de Nemours and Company
- Applicants: E I du Pont de Nemours and Company; Eileen Stieglitz
- Inventors: Perry G. Caimi; William D Hitz; Paul V Viitanen; Barry Stieglitz
- Web page: https://patentgrep.com/patent/US8569458B2

## CPC

- C12N 15/74: Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N 1/20: Bacteria; Culture media therefor
- C12P 7/065: {with microorganisms other than yeasts}
- C12P 7/10: substrate containing cellulosic material
- Y02E 50/10: Biofuels, e.g. bio-diesel
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

## Abstract

Xylose-utilizing, ethanol producing strains of Zymomonas mobilis with improved performance in medium comprising biomass hydrolysate were isolated using an adaptation process. Independently isolated strains were found to have independent mutations in the same coding region. Mutation in this coding may be engineered to confer the improved phenotype.

## Claims

### Claim 1

A polypeptide having the amino acid sequence selected from the group consisting of SEQ ID NO: 3 and SEQ ID NO:4.

### Claim 2

A method of the production of ethanol comprising:
a) providing a recombinant Zymomonas comprising a polvpeptide having the amino acid sequence selected from the group consisting of SEQ ID NO: 3 and SEQ ID NO:4
b) providing a biomass hydrolysate medium comprising xylose; and
c) growing the Zymomonas of a) in the biomass hydrolysate medium of b) wherein ethanol is produced.