Back to results
US 8,569,453 B2Granted
Fluorene compound
AssigneeAjinomoto Co., Inc.
InventorDaisuke Takahashi
Life of this patent
Estimating the term…
Abstract
Particular compounds having a fluorene skeleton are superior in broad utility and stability, as a protecting reagent for liquid phase synthesis of amino acids and/or peptides.
Claims4
A method of producing a peptide by a liquid phase synthesis process, comprising:
- (a) binding a fluorene compound to an amino acid or a peptide, to obtain a bonded product;
- (b) precipitating said bonded product and;
- (c) deprotecting said bonded product after a reaction to produce a peptide;
- wherein said fluorene compound is represented by formula (I):
- wherein ring A is an aromatic ring;
- Y is a group reactive with one or more groups selected from an amino group, a carboxyl group, and a mercapto group;
- at least one of rings A, B, and C has an organic group having an aliphatic hydrocarbon group;
- and rings A, B, and C each independently optionally have an electron withdrawing group.
5 more elements
A method of producing a peptide by a liquid phase synthesis process, comprising:
- (a) obtaining a C-fluorene compound-protected amino acid or a C-fluorene compound-protected peptide by condensing a fluorine compound with the C-terminal of a first N-protected amino acid or a first N-protected peptide;
- (b) removing a protecting group of the N-terminal of the amino acid or peptide obtained in the above-mentioned step to obtain an N-deprotected amino acid or peptide;
- (c) condensing the N-terminal of the N-deprotected amino acid or peptide with a second N-protected amino acid or a second N-protected peptide, to obtain a peptide;
- and
- (d) precipitating the peptide obtained in the above-mentioned step;
- wherein said fluorene compound is represented by formula (I):
- wherein ring A is an aromatic ring:
- Y is a group reactive with one or more groups selected from an amino group, a carboxyl group, and a mercapto group;
- at least one of rings A, B, and C has an organic group having an aliphatic hydrocarbon group;
- and rings A, B, and C each independently optionally have an electron withdrawing group.
7 more elements
Dependencies are inferred from the claim text.