# US 8,569,451 B2 · Affinity material > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,569,451 B2 - Document ID: US8569451B2 - Status: Granted - Kind: B2 - Application number: 13/263,954 - Filed: 2010-04-09 - Published: 2012-04-19 - Granted: 2013-10-29 - Earliest claimed: 2009-04-15 - Assignees: Biotage AB - Applicants: Anthony Rees; Börje Sellergren; Ecevit Yilmaz; Thomas Schrader - Inventors: Anthony Rees; Börje Sellergren; Ecevit Yilmaz; Thomas Schrader - Web page: https://patentgrep.com/patent/US8569451B2 ## CPC - C07K 17/02: Peptides being immobilised on, or in, an organic carrier - B01D 15/3809: {of the antigen-antibody type, e.g. protein A, G or L chromatography} - B01D 15/3852: {using imprinted phases or molecular recognition} - B01J 20/26: Synthetic macromolecular compounds - B01J 20/268: {Polymers created by use of a template, e.g. molecularly imprinted polymers} - C07K 16/065: {Purification, fragmentation} - C07K 17/14: Peptides being immobilised on, or in, an inorganic carrier - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28) - B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS ## Abstract The present application relates to an affinity material useful in antibody purification. ## Claims ### Claim 1 A molecularly imprinted polymer, wherein said polymer is cross-linked and comprises one or more sites selectively binding one or more antibodies, wherein said one or more sites are complementary to one or more template molecule selected from one or more peptides comprising an amino acid sequence corresponding to a subsequence of a domain of one or more antibodies wherein said amino acid sequence is selected from the group consisting of QKSLSLSPGK, QKSLSLSPG, QKSLSLSP, QKSLSLS, QKSLSL, QKSLS, QKSL, QKS, QK KSLSLSPGK, SLSLSPGK, LSLSPGK, SLSPGK, LSPGK, SPGK, PGK or GK. ### Claim 2 A molecularly imprinted polymer, obtainable by providing one or more monomers, template molecules, initiators and cross-linking agents; polymerizing said one or more monomers, templates, initiators and cross-linking agents; removing said templates; wherein said one or more template molecules is independently selected from one or more peptides comprising an amino acid sequence corresponding to a subsequence of a domain of one or more antibodies wherein said amino acid sequence is selected from the group consisting of QKSLSLSPGK, QKSLSLSPG, QKSLSLSP, QKSLSLS, QKSLSL, QKSLS, QKSL, QKS, QK KSLSLSPGK, SLSLSPGK, LSLSPGK, SLSPGK, LSPGK, SPGK, PGK or GK. ### Claim 3 (depends on 1, 2) A molecularly imprinted polymer according to any one of claims 1-2 wherein said amino acid sequence is QKSLSLSPGK. ### Claim 4 (depends on 1, 2) A molecularly imprinted polymer according to any one of claims 1-2, wherein the one or more template molecules are attached to a disposable support material or a polymer chain. ### Claim 5 (depends on 4) A molecularly imprinted polymer according to claim 4, wherein the disposable support material is silica and the polymer chain is a polyethylene glycol chain. ### Claim 6 (depends on 2) A molecularly imprinted polymer according to claim 2, wherein the monomer is a host-monomer capable of binding an amino acid side chain. ### Claim 7 (depends on 6) A molecularly imprinted polymer according to claim 6, wherein the host-monomer is selected from: ### Claim 8 (depends on 7) A molecularly imprinted polymer according to claim 7, wherein the host-monomer is selected from: ### Claim 9 (depends on 2) A molecularly imprinted polymer according to claim 2, wherein the monomer is selected from: ### Claim 10 (depends on 1, 2) A molecularly imprinted polymer according to any one of claims 1-2, wherein the molecularly imprinted polymer is grafted to a non-disposable support material. ### Claim 11 (depends on 10) A molecularly imprinted polymer according to claim 10, wherein the non-disposable support material is selected from wide pore silica, controlled pore glass and non-porous silica and polymeric supports. ### Claim 12 (depends on 1, 2) A method for purifying an antibody, wherein said antibody is contacted by a molecularly imprinted polymer according to any one of claims 1-2.