Aliphatic amino acid biosynthesis inhibitors and a method of synthesizing the same

A method of synthesizing an aliphatic amino acid biosynthesis inhibitor composition having an antifungal activity comprising the steps of:

• preparing a solution A, wherein the solution A is prepared by mixing a compound E and a compound F, wherein the compound E is dicyclohexylcarbodiimide (DCC) and the compound F is Dimethylaminopyridine (DMAP);
• preparing a solution B, wherein the solution B is prepared by mixing a compound containing a hydroxyl group, an acetylene carboxylic acid and a solvent;
• adding a prepared solution A to a prepared solution B to obtain a first mixture, wherein the prepared solution A is added to the prepared solution B at a predetermined temperature range drop wise over a predetermined time period, wherein the predetermined temperature range is 0° C.-4° C., wherein the predetermined time period is 1 hr;
• stirring the obtained first mixture for a predetermined time to obtain a residue, wherein the predetermined time is 5 hrs;
• dissolving the residue in the solvent at a predefined temperature to obtain a second mixture, wherein the predefined temperature is 0° C.;
• stirring the second mixture with a catalyst for a predefined time, wherein the predefined time is 30 minutes;
• and
• evaporating a stirred second mixture to obtain a compound, wherein the compound is hex-2-en-4-yn-1,6-dioate derivative compound.