Stabilized fibronectin domain compositions, methods and uses
AssigneeJanssen Biotech, Inc.
InventorSteven Jacobs
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Abstract
A protein scaffold based on a consensus sequence of fibronectin type III (FN3) proteins, such as the tenth FN3 repeat from human fibronectin (human Tenascin), including isolated nucleic acids that encode a protein scaffold, vectors, host cells, and methods of making and using thereof, exhibit enhanced thermal and chemical stability while presenting six modifiable loop domains which can be engineered to form a binding partner capable of binding to a target for applications in diagnostic and/or therapeutic compositions, methods and devices.
Claims4
A method of constructing a library of a scaffold-based protein derived from a stability enhanced consensus sequence of a fibronectin type III domain incorporating randomized codons in order to produce polypeptide variants, comprising the steps of:
- providing a polynucleotide sequence encoding a polypeptide comprising a stability enhanced consensus sequence of a fibronectin type III domain selected from the group consisting of SEQ ID NOs:
- 142-151;
- introducing randomizing codons into the polynucleotide sequence at selected positions;
- and
- propagating copies of the polynucleotide sequence to form a library of polynucleotides encoding variant scaffold proteins.
Dependencies are inferred from the claim text.