# US 8,569,075 B2 · Radioimmunoassay with a 96-welled micro-plate

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- Number: US 8,569,075 B2
- Document ID: US8569075B2
- Status: Granted
- Kind: B2
- Application number: 13/232,928
- Filed: 2011-09-14
- Published: 2013-03-14
- Granted: 2013-10-29
- Earliest claimed: 2011-09-14
- Assignees: Atomic Energy Council Energy—Institute of Nuclear Energy Research
- Applicants: Meng-Jun Fu; Ping-Hung Yu; Chin-Yan Tsai; Kuan-Yin Chen; Chia-Chieh Chen
- Inventors: Meng-Jun Fu; Ping-Hung Yu; Chin-Yan Tsai; Kuan-Yin Chen; Chia-Chieh Chen
- Web page: https://patentgrep.com/patent/US8569075B2

## CPC

- G01N 33/5306: {Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding}
- G01N 33/54306: {Solid-phase reaction mechanisms}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A method for using a multi-welled micro-plate in radioimmunoassay (“RIA”) is disclosed to improve the performance of RIA. At first, there is provided a multi-welled micro-plate that can be dismantled and divided into multiple wells. Then, samples are filled into the wells of the multi-welled micro-plate for incubation. Washing, tracer-adding, incubation, and washing are executed. At a final step, the multi-welled micro-plate is separated into wells, and each of to the wells is put into a test tube for gamma counting by a gamma counter.

## Claims

### Claim 1

A method for radioimmunoassay for measuring concentrations of a target material in samples including the steps of:
providing a multi-welled micro-plate 21 that can be dismantled;
executing radioimmunoassay including the steps of:
filling an antibody 31, specific for the target material, in the wells 22 of the multi-welled micro-plate 21 so that the antibody 31 is coated on an internal side of each of the wells 22 of the multi-welled micro-plate 21;
washing away an excessive portion of the antibody 31 from the wells 22;
coating an blocking reagent 32 on a portion of the surface of the multi-welled micro-plate 21 that is not covered by the antibody 31;
removing an excessive portion of the blocking reagent 32;
filling samples 33 of a target material in the wells 22;
oscillating the multi-welled micro-plate 21 for a predetermined period of time;
washing away an excessive portion of the samples 33 is by washing buffer for several times;
labeling an antibody 34 specific for the target material with iodine-125 and filling it in the wells 22;
oscillating the multi-welled micro-plate 21 for another predetermined period of time;
washing away an excessive portion of the antibody 34 labeled with the iodine-125 by washing buffer for several times; and
inverting and locating the multi-welled micro-plate 21 on a tower of tissue paper so that all liquid leaves the wells 22; and
executing a gamma counting including the steps of:
dismantling the multi-welled micro-plate 21 into separate wells 22;
casting each of the wells 22 in a test tube 23; and
providing a gamma counter 24 is used for the gamma counting in the test tubes 23, thus measuring the concentrations of the target material in the samples 33.