# US 8,569,064 B2 · Manipulation of flavonoid biosynthesis in plants

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- Number: US 8,569,064 B2
- Document ID: US8569064B2
- Status: Granted
- Kind: B2
- Application number: 12/844,615
- Filed: 2010-07-27
- Published: 2011-03-03
- Granted: 2013-10-29
- Earliest claimed: 2001-10-05
- Assignees: Agriculture Victoria Services PTY LTD; AgResearch Limited
- Applicants: German Spangenberg; Timothy Ivor Sawbridge; Eng-Kok Ong; Michael Emmerling
- Inventors: German Spangenberg; Timothy Ivor Sawbridge; Eng-Kok Ong; Michael Emmerling
- Web page: https://patentgrep.com/patent/US8569064B2

## CPC

- C12N 9/93: {Ligases (6)}
- A61P 25/28: for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
- C12N 15/52: Genes encoding for enzymes or proenzymes
- C12N 15/825: {involving pigment biosynthesis}
- C12N 15/8261: {with agronomic (input) traits, e.g. crop yield}
- C12N 15/8279: {for biotic stress resistance, pathogen resistance, disease resistance}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present invention relates to nucleic acids and nucleic acid fragments encoding amino acid sequences for flavonoid biosynthetic enzymes in plants, and the use thereof for the modification of flavonoid biosynthesis in plants. More particularly, the flavonoid biosynthetic enzyme is selected from the group consisting of chalcone isomerase (CHI), chalcone synthase (CHS), chalcone reductase (CHR), dihydroflavonol 4-reductase (DFR), leucoanthocyanidin reductase (LCR), flavonoid 3′,5′ hydrolase (F3′5′H), flavanone 3-hydroxylase (F3H), flavonoid 3′-hydroxylase (F3′H), phenylalanine ammonia-olyase (PAL) and vestitone reductase (VR), and functionally active fragments and variants thereof.

## Claims

### Claim 1

A substantially purified or isolated nucleic acid, said nucleic acid being selected from the group consisting of:
(a) a nucleotide sequence selected from the group consisting of SEQ ID Nos: 185 and 187 to 193;
(b) a nucleotide sequence comprising at least bases 1 to 311 of Seq ID No. 193,
(c) a nucleotide sequence which is the full length complement to a nucleotide sequence selected from the group consisting of SEQ ID Nos: 185 and 187 to 193; and
(d) a probe nucleotide sequence, wherein the entire probe nucleotide sequence is the same as or complementary to a portion of a target sequence, said target sequence having a sequence as defined in paragraph (a), wherein the probe nucleotide sequence has a length sufficient for sequence specific hybridization with the target sequence.

### Claim 2 (depends on 1)

A construct including the nucleic acid of claim 1.

### Claim 3 (depends on 2)

A plant cell, plant, plant seed or other plant part including the construct of claim 2, wherein the construct is an expression vector.

### Claim 4 (depends on 1)

A method selected from the group consisting of:
(a) modifying flavonoid biosynthesis in a plant;
(b) modifying protein binding, metal chelation, anti-oxidation, and/or UV-light absorption in a plant;
(c) modifying pigment production in a plant;
(d) modifying plant defense to a biotic stress; and
(e) modifying forage quality of a plant by disputing protein foam and/or conferring protection from rumen pasture bloat;
said method including introducing into said plant an effective amount of the nucleic acid of claim 1.

### Claim 5 (depends on 1)

The nucleic acid of claim 1, wherein the nucleic acid sequence comprises 1-311 of Seq ID No. 193.

### Claim 6 (depends on 1)

The nucleic acid of claim 1 wherein the nucleic acid is a probe having a size of at least 20 nucleotides.

### Claim 7 (depends on 1)

The nucleic acid of claim 1, wherein the nucleic acid is the full length complement to a nucleotide sequence selected from the group consisting of SEQ ID Nos: 185 and 187 to 193.

### Claim 8 (depends on 1)

The nucleic acid of claim 1, wherein the nucleic acid has a nucleotide sequence selected from the group consisting of SEQ ID Nos: 185 and 187 to 193.