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US 8,569,062 B2Granted
Cryogels of PVA-boronic acid containing co-polymers for cell culture
AssigneeIndian Institute of Technology Kanpur
InventorAshok Kumar
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Abstract
The present invention relates to a cryogel which contains a polyol selected from polyvinyl alcohol or galactomannan and a co-polymer of Formula I: The cryogel is formed by mixing the monomers of the co-polymer of Formula I at a temperature of less about 5 degrees C. with the polyol and a free radical initiator in an aqueous solution and polymerizing the solution to form the cryogel. The cryogel is used for culturing cells and can be decomposed by contact with a monosaccharide.
Claims16
A cryogel comprising a polyol and a co-polymer of Formula I:
- wherein,
- m is from 150000 to 220000;
- n is from 4000 to 6000;
- p is 0 or from 3500 to 5500;
- x is from 1 to 5;
- L is a bond or —CONH— wherein the nitrogen is attached to the boron-substituted phenyl group;
- R1, R2, R3, and R4 are independently H or C1-C6 alkyl;
- each R5 independently is H or C1-C3 alkyl;
- and
- either X1 is H and X2 is —B(OH)2, or X is —B(OH)2 and X2 is H;
- wherein the polyol is polyvinyl alcohol or galactomannan.
8 more elements
A method of preparing an aqueous mixture comprising:
- mixing at a temperature of less than about 5° C., a polyol, a radical initiator, a compound of Formula II, a compound of Formula III, and optionally a compound of Formula IV to form the aqueous mixture:
- wherein
- L is a bond or —CONH— wherein the nitrogen is attached to the boron-substituted phenyl group;
- R1, R2, R3, and R4 are independently H or C1-C6 alkyl;
- x is from 1 to 5;
- each R5 independently is H or C1-C3 alkyl;
- and
- either X1 is H and X2 is —B(OH)2, or X1 is —B(OH)2 and X2 is H;
- wherein the polyol is polyvinyl alcohol or galactomannan.
6 more elements
Dependencies are inferred from the claim text.