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US 8,569,053 B2Granted
In-vitro fertilization systems with spermatozoa separated into X-chromosome and Y-chromosome bearing populations
AssigneeXy, LLC
InventorsGeorge E. Seidel, Kehuan Lu, Tae Kwang Suh
Life of this patent
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Abstract
An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).
Claims18
A method of in-vitro fertilization of oocytes, comprising:
- a) obtaining oocytes of a non-human mammal;
- b) obtaining stained sperm cells of said non-human mammal, said stained sperm cells separated by a flow cytometer into an X-chromosome bearing population or a Y-chromosome bearing population;
- c) combining said oocytes in a fertilization medium with said stained sperm cells separated by said flow cytometer, said stained sperm cells of either one of said X-chromosome bearing population or said Y-chromosome bearing population;
- and
- d) fertilizing in-vitro at least one of said oocytes with said stained sperm cells of either one said X-chromosome bearing population or said Y-chromosome bearing population to produce sex-selected embryos capable of blastocyst formation.
2 more elements
Dependencies are inferred from the claim text.