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US 8,569,041 B2Granted
Multiplex automated genome engineering
AssigneePresident and Fellows of Harvard College
InventorsGeorge M. Church, Harris H. Wang, Farren J. Isaacs
Life of this patent
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Abstract
The present invention relates to automated methods of introducing multiple nucleic acid sequences into one or more target cells.
Claims13
An apparatus for introducing multiple nucleic acid sequences into a cell comprising
- a receptacle containing one or more cells and optionally containing transformation or transfection media;
- fluidics and automatically controlled valves and pumps to introduce one or more nucleic acid oligomers and optionally transformation or transfection media into the receptacle;
- fluidics and automatically controlled valves and pumps to replace transformation or transfection media with growth media in the receptacle;
- and
- fluidics and automatically controlled valves and pumps to pass ambient air or fluid through a jacket to incubate the cells in the receptacle.
2 more elements
An apparatus for introducing multiple nucleic acid sequences into a cell comprising
- a series of receptacles containing one or more cells and optionally containing transformation or transfection media;
- fluidics and automatically controlled valves and pumps to introduce one or more nucleic acid oligomers and optionally transformation or transfection media into the series of receptacles;
- fluidics and automatically controlled valves and pumps to replace transformation or transfection media with growth media in the series of receptacles;
- and
- fluidics and automatically controlled valves and pumps to pass ambient air or fluid through a jacket to incubate the cells in the series of receptacles.
2 more elements
Dependencies are inferred from the claim text.