# US 8,569,039 B2 · Ethanol resistance Saccharomyces cerevisiae GP-01 by protoplast fusion, method for manufacturing thereof, method for manufacturing yeast containing high content of bio organic germanium by using Saccharomyces cerevisiae GP-01 and high water soluble sodium metagermanate as a germanium source

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- Number: US 8,569,039 B2
- Document ID: US8569039B2
- Status: Granted
- Kind: B2
- Application number: 13/132,360
- Filed: 2008-12-31
- Published: 2011-09-22
- Granted: 2013-10-29
- Earliest claimed: 2008-12-04
- Assignees: Geranti Pharm Ltd
- Applicants: Tsang-Uk Sohn; Dae-Heoun Baek
- Inventors: Tsang-Uk Sohn; Dae-Heoun Baek
- Web page: https://patentgrep.com/patent/US8569039B2

## CPC

- C12N 1/16: Yeasts; Culture media therefor
- A23L 33/14: Yeasts or derivatives thereof
- A23L 33/165: Complexes or chelates
- C12N 1/165: Yeast isolates
- C12N 1/185: Saccharomyces isolates
- C12N 15/04: Fungi
- C12R 2001/72: Candida
- C12R 2001/865: Saccharomyces cerevisiae
- Y02E 50/10: Biofuels, e.g. bio-diesel
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

## Abstract

A biologically pure culture of Saccharomyces cervisiae GP-01, and a method for producing Saccharomyces Cervisiae GP-01 which is ethanol-resistant and obtained by protoplast fusing Saccharomyces Cerevisiae (KCTC 7904) and Candida Ethanolica (KCTC 7181). Further, the present invention provides yeast containing the high content of organic bio-germanium (the yeast Ge-32K) and a method for producing the yeast Ge-32K, comprising adding the yeast GP-01 into a solution of sodium metagermanate (Na2GeO3) at the volume ratio of 1:0.5˜2; adding 0.1˜0.4 wt % of surfactant, instead of germanium dioxide as used in the prior arts; and cultivating an obtained broth. The obtained yeast Ge-32K contains the higher content of the organic bio-germanium than the conventional yeast as produced by using germanium dioxide.

## Claims

### Claim 1

A method for preparing a yeast Ge-32K comprising adding Saccharomyces cerevisiae GP-01, which was deposited with the Korea Collection for Type Culture of Korea Research Institute of Bioscience and Biotechnology under the accession number KCTC 11399BP, to a culture medium containing sodium metagermanate (Na2GeO3) and culturing the Saccharomyces cerevisiae GP-01 in the culture medium containing the sodium metagermanate (Na2GeO3).

### Claim 2 (depends on 1)

The method according to claim 1, wherein the sodium metagermanate (Na2GeO3) is prepared by (i) dissolving either sodium carbonate (Na2CO3), calcium carbonate (CaCO3) or potassium bicarbonate (KHCO3) in water and adding germanium dioxide (GeO2) to the solution prepared by said (i) at an equivalent volume ratio.

### Claim 3 (depends on 1)

The method according to claim 1, wherein a mixing volume ratio between the Saccharomyces cervisiae GP-01 and the culture medium containing the sodium metagermanate (Na2GeO3) is 1:0.5-2.0.

### Claim 4 (depends on 2)

The method according to claim 2, wherein the culturing is performed for 19˜21 hours at pH 9˜10 and temperature 30˜40° C.