# US 8,569,030 B2 · Type II restriction-modification system methylation subunit of Alicyclobacillus acidocaldarius

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- Number: US 8,569,030 B2
- Document ID: US8569030B2
- Status: Granted
- Kind: B2
- Application number: 13/604,979
- Filed: 2012-09-06
- Published: 2013-01-31
- Granted: 2013-10-29
- Earliest claimed: 2010-05-05
- Assignees: Battelle Energy Alliance, LLC
- Applicants: Brady D. Lee; Deborah T. Newby; Jeffrey A. Lacey; David N. Thompson; Vicki S. Thompson; William A. Apel; Francisco F. Roberto; David W. Reed
- Inventors: Brady D. Lee; Deborah T. Newby; Jeffrey A. Lacey; David N. Thompson; Vicki S. Thompson; William A. Apel; Francisco F. Roberto; David W. Reed
- Web page: https://patentgrep.com/patent/US8569030B2

## CPC

- C12N 15/74: Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C07K 14/195: from bacteria
- C12N 9/00: Enzymes; Proenzymes; Compositions thereof (preparations containing enzymes for cleaning teeth A61K8/66, A61Q11/00; medicinal preparations containing enzymes or proenzymes A61K38/43; enzyme containing detergent compositions C11D; {enzymes with nucleic acid structure, e.g. ribozymes, C12N15/113}); Processes for preparing, activating, inhibiting, separating or purifying enzymes (preparation of malt C12C1/00)
- C12N 9/1007: {Methyltransferases (general) (2.1.1.)}
- C12N 9/52: derived from bacteria {or Archaea}
- C12N 15/87: Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12Y 201/01072: Site-specific DNA-methyltransferase (adenine-specific) (2.1.1.72)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12Y: ENZYMES

## Abstract

Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering recombination inside or outside of a cell using isolated and/or purified polypeptides and/or nucleic acid sequences from Alicyclobacillus acidocaldarius.

## Claims

### Claim 1

An isolated nucleic acid sequence comprising a nucleic acid sequence encoding a polypeptide having at least 90% sequence identity to SEQ ID NO: 2976.

### Claim 2 (depends on 1)

The isolated nucleic acid sequence of claim 1, wherein the polypeptide has activity at or below about pH 8.

### Claim 3 (depends on 1)

The isolated nucleic acid sequence of claim 1, wherein the polypeptide has activity at a temperature at or above about 50 degrees Celsius.

### Claim 4 (depends on 1)

The isolated nucleic acid sequence of claim 1, wherein the nucleic acid sequence is present in a vector.

### Claim 5 (depends on 1)

The isolated nucleic acid sequence of claim 1, wherein the polypeptide has activity as a Type II restriction-modification system methylation subunit.

### Claim 6 (depends on 1)

The isolated nucleic acid sequence of claim 1, wherein the polypeptide comprises SEQ ID NO: 2976.

### Claim 7 (depends on 1)

The isolated nucleic acid sequence of claim 1, wherein the nucleic acid sequence comprises SEQ ID NO: 2977.