# US 8,569,028 B2 · Method for encapsulation of microparticles

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- Number: US 8,569,028 B2
- Document ID: US8569028B2
- Status: Granted
- Kind: B2
- Application number: 12/797,087
- Filed: 2010-06-09
- Published: 2011-12-15
- Granted: 2013-10-29
- Earliest claimed: 2010-06-09
- Assignees: The United States of America as represented by the Secretary of Agriculture
- Applicants: Xixuan Jin; Daniel B. Custis
- Inventors: Xixuan Jin; Daniel B. Custis
- Web page: https://patentgrep.com/patent/US8569028B2

## CPC

- C12N 1/04: Preserving or maintaining viable microorganisms (immobilised microorganisms C12N11/00)
- B01J 13/043: {Drying and spraying}
- C12N 1/14: Fungi (culture of mushrooms A01G18/00; as new plants A01H15/00); Culture media therefor
- A61K 2035/128: {capsules, e.g. microcapsules}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

A method for encapsulation of microparticles (e.g., fungal conidia), involving (i) suspending microparticles in an aqueous solution containing at least one sugar to form an aqueous suspension wherein the concentration of said sugar is about 0.1 to 10% w/v, and (ii) spray drying said aqueous suspension with a spray dryer, wherein the inlet temperature of said spray dryer is about 40° C. to about 140° C. and the outlet temperature of said spray dryer is about 20° C. to about 80° C.

## Claims

### Claim 1

A method for encapsulation of microparticles, comprising suspending microparticles in an aqueous solution containing at least one sugar to form an aqueous suspension wherein the concentration of said sugar is about 0.1% to about 10.0% w/v, and spray drying said aqueous suspension with a spray dryer, wherein the inlet temperature of said spray dryer is about 40° C. to about 140° C. and the outlet temperature of said spray dryer is about 20° C. to about 80° C.; wherein said microparticles are fungal conidia, fungal spores, fungal hyphal cells, or mixtures thereof; wherein the amount of said sugar loaded on said encapsulated microparticle is about 2 to about 10 mg per one gram of said encapsulated microparticles.

### Claim 2 (depends on 1)

The method according to claim 1, wherein said microparticles are fungal conidia.

### Claim 3 (depends on 1)

The method according to claim 1, wherein said microparticles are Bacillus species, Entomophaga species, Paecilomyces species, Metarhizium species, Beauveria species, Penicillium species, Trichoderma species, Steinernema species, Heterorhabditis species or mixtures thereof.

### Claim 4 (depends on 1)

The method according to claim 1, wherein said microparticles are Bacillus thuringiensis, Entomophaga maimalga, Paecilomyce fumosoroseus, Metarhizium anisopliae, Beauveria bassiana, Trichoderma harzianum, or mixtures thereof.

### Claim 5 (depends on 1)

The method according to claim 1, wherein said sugar is selected from the group consisting of monosaccharides, disaccharides, polysaccharides, natural gums, molasses, glycerol, honey, corn-syrup, maple syrup, and mixtures thereof.

### Claim 6 (depends on 1)

The method according to claim 1, wherein said sugar is selected from the group consisting of glucose, fructose, galactose, xylose, ribose, sucrose, lactose, maltose, trehalose, cellobiose, starch, cellulose, natural gums, molasses, glycerol, honey, corn-syrup, maple syrup, and mixtures thereof.

### Claim 7 (depends on 1)

The method according to claim 1, wherein said inlet temperature of said spray dryer is about 50° C. to about 80° C. and said outlet temperature of said spray dryer is about 25° C. to about 45° C.

### Claim 8 (depends on 1)

The method according to claim 1, wherein said inlet temperature of said spray dryer is about 60° C. and said outlet temperature of said spray dryer is about 30° C.

### Claim 9 (depends on 1)

The method according to claim 1, wherein said microparticles have a size of from about 8 to about 30 μm.