Systems using cell culture for production of isoprene
AssigneesDanisco US Inc., The Goodyear Tire & Rubber Company
InventorsGopal K. Chotani, Joseph C. McAuliffe, Caroline M. Peres, Gregory M. Whited
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Abstract
The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity. Additionally, the invention provides methods of producing isoprene by culturing cells under conditions suitable for isoprene production while maintaining cell viability and/or metabolic activity.
Claims20
A method of producing a compound, wherein the compound has one or more characteristics selected from the group consisting of (a) a Henry's law coefficient of less than 250 M/atm and (b) a solubility in water of less than 100 g/L, the method comprising:
- a) culturing cells comprising one or more nucleic acids encoding for an isoprene synthase polypeptide;
- b) adding an oxygen-containing gas to the culture at a gas sparging rate between about 0.01 vvm to about 2 vvm, thereby producing the compound;
- and
- c) recovering the compound made in step (b).
A method of producing a compound, wherein the compound has one or more characteristics selected from the group consisting of (a) a Henry's law coefficient of less than 250 M/atm and (b) a solubility in water of less than 100 g/L, the method comprising:
- a) culturing cells comprising one or more nucleic acids encoding for an isoprene synthase polypeptide;
- b) adding an oxygen-containing gas to the culture at a gas sparging rate of at least 0.1 vvm, thereby producing the compound;
- and
- c) recovering the compound from the gas phase.
Dependencies are inferred from the claim text.