Producing dicarboxylic acids using polyketide synthases
AssigneeThe Regents of the University of California
InventorsLeonard Katz, Jeffrey L Fortman, Jay D Keasling
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Abstract
The present invention provides for a polyketide synthase (PKS) capable of synthesizing a dicarboxylic acid (diacid). Such diacids include diketide-diacids and triketide-diacids. The invention includes recombinant nucleic acid encoding the PKS, and host cells comprising the PKS. The invention also includes methods for producing the diacids.
Claims13
A method of producing a diacid, comprising culturing a prokaryotic or yeast host cell comprising a recombinant nucleic acid that encodes a polyketide synthase (PKS), said PKS composed of a loading module containing a beta-keto acyl carrier protein synthase Q domain (KSQ) lacking decarboxylase activity, one or more extender modules, and a thioesterase (TE) domain, in a suitable culture medium such that the diacid is produced.
Dependencies are inferred from the claim text.