# US 8,569,022 B2 · Extracellular aldonolactonase

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- Number: US 8,569,022 B2
- Document ID: US8569022B2
- Status: Granted
- Kind: B2
- Application number: 13/813,153
- Filed: 2011-06-23
- Published: 2013-08-01
- Granted: 2013-10-29
- Earliest claimed: 2010-07-30
- Assignees: The Regents of the University of California
- Applicants: William T. Beeson, IV; James H. Doudna Cate; Michael A. Marletta
- Inventors: William T. Beeson, IV; James H. Doudna Cate; Michael A. Marletta
- Web page: https://patentgrep.com/patent/US8569022B2

## CPC

- C12P 19/02: Monosaccharides (2-ketogulonic acid C12P7/60)
- A23K 10/14: Pretreatment of feeding-stuffs with enzymes
- A23K 50/30: for swines
- A23K 50/75: for poultry
- C11D 3/386: Preparations containing enzymes {, e.g. protease or amylase}
- C12N 9/18: Carboxylic ester hydrolases {(3.1.1)}
- C12P 7/58: Aldonic, ketoaldonic or saccharic acids (uronic acids C12P19/00)
- C12Y 101/03004: Glucose oxidase (1.1.3.4)
- C12Y 101/99018: Cellobiose oxidase (1.1.99.18)
- C12Y 301/01017: Gluconolactonase (3.1.1.17)
- D21C 5/00: Other processes for obtaining cellulose, e.g. cooking cotton linters (obtaining fibres for spinning D01C); {Processes characterised by the choice of cellulose-containing starting materials}
- C12P 2201/00: Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
- D21C 5/005: {Treatment of cellulose-containing material with microorganisms or enzymes}
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- A23K: FODDER
- C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES
- D21C: PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR

## Abstract

The present disclosure relates to hydrolysis of hexose-δ-lactones by use of an S. thermophile extracellular aldonolactonase. In particular the present disclosure relates to compositions including a S. thermophile extracellular aldonolactonase and methods of use thereof.

## Claims

### Claim 1

A method of producing aldonic acid comprising contacting a hexose-δ-lactone substrate with a recombinant polypeptide comprising a polypeptide having at least 90% sequence identity to SEQ ID NO: 1 wherein said polypeptide has lactonase activity.

### Claim 2 (depends on 1)

The method of claim 1, wherein the recombinant polypeptide comprises a polypeptide having at least 95% sequence identity to SEQ ID NO: 1.

### Claim 3 (depends on 1)

The method of claim 1, wherein the recombinant polypeptide comprises a polypeptide having at least 99% sequence identity to SEQ ID NO: 1.

### Claim 4 (depends on 1)

The method of claim 1, wherein the recombinant polypeptide comprises SEQ ID NO: 1.

### Claim 5 (depends on 1)

The method of claim 1, wherein the hexose-δ-lactone substrate is cellobiono-δ-lactone.

### Claim 6 (depends on 1)

The method of claim 1, wherein the hexose-δ-lactone substrate is glucono-δ-lactone.

### Claim 7 (depends on 1)

The method of claim 1, wherein the hexose-δ-lactone substrate is lactono-δ-lactone.

### Claim 8 (depends on 2)

The method of claim 2, wherein the hexose-δ-lactone substrate is cellobiono-δ-lactone.

### Claim 9 (depends on 2)

The method of claim 2, wherein the hexose-δ-lactone substrate is glucono-δ-lactone.

### Claim 10 (depends on 2)

The method of claim 2, wherein the hexose-δ-lactone substrate is lactono-δ-lactone.

### Claim 11 (depends on 3)

The method of claim 3, wherein the hexose-δ-lactone substrate is cellobiono-δ-lactone.

### Claim 12 (depends on 3)

The method of claim 3, wherein the hexose-δ-lactone substrate is glucono-δ-lactone.

### Claim 13 (depends on 3)

The method of claim 3, wherein the hexose-δ-lactone substrate is lactono-δ-lactone.

### Claim 14 (depends on 4)

The method of claim 4, wherein the hexose-δ-lactone substrate is cellobiono-δ-lactone.

### Claim 15 (depends on 4)

The method of claim 4, wherein the hexose-δ-lactone substrate is glucono-δ-lactone.

### Claim 16 (depends on 4)

The method of claim 4, wherein the hexose-δ-lactone substrate is lactono-δ-lactone.