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US 8,569,021 B2Granted
Materials and methods for the production of biodiesel
AssigneeUniversity of Tennessee Research Foundation
InventorsFeng Chen, Nan Zhao
Life of this patent
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Abstract
The subject invention provides novel microorganisms comprising polynucleotides and polypeptides encoding a methyltransferase. The subject invention provides novel methods for the production of biodiesel.
Claims13
A composition comprising:
- a genetically modified microorganism comprising a nucleic acid encoding SEQ ID NO:
- 2 operably linked to a promoter, and a culture medium comprising C2 to C10 fatty acids.
A method of producing methylated fatty acids comprising:
- culturing one or more genetically modified microorganism comprising a nucleic acid encoding SEQ ID NO:
- 2 operably linked to a promoter, in a culture medium comprising C2 to C10 fatty acids under conditions that allow for the methylation of said C2 to C10 fatty acids.
A method of making a biodiesel comprising:
- culturing a genetically modified microorganism comprising a nucleic acid encoding SEQ ID NO:
- 2 operably linked to a promoter, in a medium comprising:
- a crop oil, waste oil or animal fat;
- and methylating fatty acids in said medium, said fatty acids being:
- Acetic (C2) acid, Propionic (C3) acid, Butyric (C4) acid, Valeric (C5) acid, Hexanoic (C6) acid, Heptanoic (C7) acid, Caprylic (C8) acid, Nonanoic (C9) acid, or Capric (C10) acid.
2 more elements
A composition comprising:
- culture medium and a genetically modified microorganism comprising a nucleic acid encoding SEQ ID NO:
- 2 operably linked to a promoter, said culture medium comprising a crop oil, animal fats or waste oils, said crop oil, animal fats or waste oils containing C2 to C10 fatty acids.
Dependencies are inferred from the claim text.