# US 8,569,012 B2 · Method for stabilization of aqueous acrylamide solution

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- Number: US 8,569,012 B2
- Document ID: US8569012B2
- Status: Granted
- Kind: B2
- Application number: 12/922,340
- Filed: 2009-03-12
- Published: 2011-01-13
- Granted: 2013-10-29
- Earliest claimed: 2008-03-14
- Assignees: Mitsubishi Rayon Co., Ltd.
- Applicants: Masahito Oda; Katsuo Ishii
- Inventors: Masahito Oda; Katsuo Ishii
- Web page: https://patentgrep.com/patent/US8569012B2

## CPC

- C07C 231/22: Separation; Purification; Stabilisation; Use of additives (separation of optical isomers C07C231/20)
- C12N 1/205: Bacterial isolates
- C12P 13/02: Amides, e.g. chloramphenicol {or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes (peptides C12P21/00 or C07K)}
- C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales}
- C12R 2001/19: Escherichia coli
- C07C: ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

The present invention provides a method for conveniently stabilizing an aqueous acrylamide solution using a stabilizer which can be separated and removed easily. In the method for stabilizing an aqueous acrylamide solution according to the present invention, a microbial cell is added to the aqueous acrylamide solution at a dried cell weight concentration of 1 to 14000 mg/L.

## Claims

### Claim 1

A method for preventing polymerization of an aqueous acrylamide solution during preservation and/or transportation of the acrylamide solution comprising:
adding a microbial cell having nitrile hydratase activity to the aqueous acrylamide solution at a dried cell weight concentration of 1 to 14000 mg/L, wherein the microbial cell having nitrile hydratase activity is selected from the group consisting of Rhodococcus rhodochrous, Escherichia coli MT-10822 (Accession number: FERM BP-5785), and mixtures thereof, and
preserving and/or transporting the acrylamide solution in the presence of the microbial cell,
wherein the presence of the microbial cell in the acrylamide solution prevents polymerization of the aqueous acrylamide solution.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the dried cell weight concentration is 10 to 14000 mg/L.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the Rhodococcus rhodochrous is selected from the group consisting of Rhodococcus rhodochrousJ-1 (Accession number: FERM BP-1478), Rhodococcus rhodochrous M8 (Accession number: VKPMB-S926), and mixtures thereof.

### Claim 4 (depends on 1)

The method according to claim 1 wherein the microbial cell is added at the time of preparation of the aqueous acrylamide solution from an aqueous acrylonitrile solution.

### Claim 5 (depends on 1)

The method according to claim 1, wherein the nitrile hydratase activity is an activity which produces an acrylamide from an acrylonitrile.

### Claim 6 (depends on 1)

The method according to claim 1, wherein the acrylamide has been produced from an acrylonitrile using the microbial cell having nitrile hydratase activity.

### Claim 7 (depends on 1)

The method of claim 1, wherein the microbial cell is added after preparation of the aqueous acrylamide solution.

### Claim 8

A method for reducing foamability of an acrylamide solution during transportation, concentration, distillation, and/or crystallization of the acrylamide solution comprising:
adding a microbial cell having nitrile hydratase activity to the aqueous acrylamide solution at a dried cell weight concentration of 500 mg/L or lower, and
transporting, concentrating, distilling, and/or crystallizing the acrylamide solution in the presence of the microbial cell,
wherein the microbial cell having nitrile hydratase activity is selected from the group consisting of Rhodococcus rhodochrous, Escherichia coil MT-10822 (Accession number: FERM BP-5785), and mixtures thereof, and
wherein the presence of the microbial cell in the acrylamide solution reduces foamability of the aqueous acrylamide solution.