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US 8,568,987 B2Granted
Chemotaxis diagnostic
AssigneeGlycotope Biotechnology GmbH
InventorsCarl Thomas Nebe, Karin Hartmann
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Abstract
A method is described for determining the chemotactic activity of leukocytes in a sample, in which method the expression of a cell adhesion molecule is determined.
Claims12
A method for diagnosing an immunological defect or a disturbance in leukocyte function in a subject, comprising:
- (i) providing a leukocyte containing sample from said subject;
- (ii) pre-incubating said sample with a chemotaxis stimulant;
- (iii) conducting said leukocytes through a porous membrane wherein said porous membrane is coated with collagen, fibrinogen or gelatin;
- (iv) determining in a flow-through cytometer;
- (a) any change in shape or size of the leukocytes, and
- (b) the number of leukocytes which have migrated through said porous membrane;
- and
- (v) comparing the results from (iv) to a control, wherein differences therebetween indicate an immunological defect or a disturbance in leukocyte function in said subject.
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Dependencies are inferred from the claim text.