# US 8,568,985 B2 · Breast endothelial cell expression patterns > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,568,985 B2 - Document ID: US8568985B2 - Status: Granted - Kind: B2 - Application number: 13/176,222 - Filed: 2011-07-05 - Published: 2011-10-27 - Granted: 2013-10-29 - Earliest claimed: 2003-04-01 - Assignees: Genzyme Corporation; The Johns Hopkins University - Applicants: Saraswati Sukumar; Stephen L. Madden - Inventors: Saraswati Sukumar; Stephen L. Madden - Web page: https://patentgrep.com/patent/US8568985B2 ## CPC - C07K 16/18: against material from animals or humans - A61K 38/17: from animals; from humans {(enzyme inhibitors A61K38/005)} - A61K 39/0011: {Cancer antigens} - A61K 39/39558: {against tumor tissues, cells, antigens} - A61K 51/1018: {against material from animals or humans} - A61P 35/00: Antineoplastic agents - A61P 37/04: Immunostimulants - A61P 43/00: Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 - C12Q 1/6886: for cancer (immunoassay for cancer G01N33/575) - G01N 33/5011: {for testing antineoplastic activity} - G01N 33/57515: of the breast - C07K 2317/34: Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues - C12Q 2600/136: Screening for pharmacological compounds - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract To gain a better understanding of breast tumor angiogenesis, breast endothelial cells (ECs) were isolated and evaluated for gene expression patterns. When transcripts from breast ECs derived from normal and malignant breast tissues were compared, genes that were specifically elevated in tumor-associated breast endothelium were revealed. These results confirm that neoplastic and normal endothelium in human breast are distinct at the molecular level, and have significant implications for the development of anti-angiogenic therapies in the future. ## Claims ### Claim 1 A method to aid in diagnosing breast tumor, comprising the steps of: detecting an expression product of at least one gene in a first breast tissue sample suspected of being neoplastic wherein said one gene is snail homolog 1 (Drosophila); comparing expression of the at least one gene in the first breast tissue sample with expression of the at least one gene in a second breast tissue sample which is normal and identifying the first breast tissue sample as likely to be neoplastic when expression of the one gene in the first breast tissue sample is increased relative to the second tissue sample. ### Claim 2 (depends on 1) The method of claim 1 wherein the increased expression of the at least one gene in the first breast tissue sample relative to the second tissue sample is at least two-fold higher. ### Claim 3 (depends on 1) The method of claim 1 wherein the increased expression of the at least one gene in the first breast tissue sample relative to the second tissue sample is at least five-fold higher. ### Claim 4 (depends on 1) The method of claim 1 wherein the increased expression of the at least one gene in the first breast tissue sample relative to the second tissue sample is at least ten-fold higher. ### Claim 5 (depends on 1) The method of claim 1 wherein the expression product is RNA. ### Claim 6 (depends on 1) The method of claim 1 wherein the expression product is protein. ### Claim 7 (depends on 1) The method of claim 1 wherein the first and second tissue samples are from a human. ### Claim 8 (depends on 1) The method of claim 1 wherein the first and second tissue samples are from the same human. ### Claim 9 (depends on 1) The method of claim 1 wherein the step of detecting is performed using a Western blot. ### Claim 10 (depends on 1) The method of claim 1 wherein the step of detecting is performed using an immunoassay. ### Claim 11 (depends on 1) The method of claim 1 wherein the step of detecting is performed using an immunohistochemical assay. ### Claim 12 (depends on 1) The method of claim 1 wherein the step of detecting is performed using SAGE. ### Claim 13 (depends on 1) The method of claim 1 wherein the step of detecting is performed using hybridization to a microarray.