# US 8,568,818 B2 · High-purity Rebaudioside D and low-calorie carbonated drink containing the same

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- Number: US 8,568,818 B2
- Document ID: US8568818B2
- Status: Granted
- Kind: B2
- Application number: 12/785,506
- Filed: 2010-05-24
- Published: 2011-04-21
- Granted: 2013-10-29
- Earliest claimed: 2009-10-15
- Assignees: Pure Circle Sdn Bhd
- Applicants: Varuzhan Abelyan; Avetik Markosyan; Lidia Abelyan
- Inventors: Varuzhan Abelyan; Avetik Markosyan; Lidia Abelyan
- Web page: https://patentgrep.com/patent/US8568818B2

## CPC

- A23G 9/42: containing plants or parts thereof, e.g. fruits, seeds, extracts (containing gums A23G9/34)
- A23L 2/60: Sweeteners
- A23L 27/36: {Terpene glycosides}
- A23L 27/50: Soya sauce
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- A23G: COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION

## Abstract

The invention provides methods of purifying Rebaudioside D from the Stevia rebaudiana Bertoni plant extract along with Rebaudioside A. The methods are useful for producing high purity Rebaudioside D and Rebaudioside A. The invention further provides a low-calorie carbonated drink containing the purified Rebaudioside D and a process for making the low-calorie carbonated drink containing the purified Rebaudioside D.

## Claims

### Claim 1

A low-calorie carbonated drink, comprising:
a natural sweetener composition comprising Rebaudioside D in the range of 81-96 wt %, Rebaudioside A in the range of 3-16 wt %, Stevioside in the range of 0.1-0.8 wt %, Rebaudioside C in the range of 0.1-0.7 wt %, Rebaudioside E in the range of 0.2-0.5 wt %, Rebaudioside F in the range of 0.1-0.2 wt %;
cola flavor;
phosphoric acid;
citric acid;
carbonated water; and
one or more beverage ingredients;
whereby all components are admixed so as to produce the low-calorie carbonated drink.

### Claim 2 (depends on 1)

The low-calorie carbonated drink of claim 1, wherein the natural sweetener composition is obtained from Stevia rebaudiana Bertoni plant by a method comprising the following steps:
a) providing an extract of Stevia rebaudiana Bertoni plant;
b) dissolving the extract in a first aqueous solution of organic solvent to result in a first mixture of steviol glycosides, wherein the organic solvent is selected from the group consisting of methanol, ethanol, 1-propanol, isopropanol, and a mixture thereof, and the organic solvent is 75-99 vol. %;
c) inducing crystallization in the first mixture;
d) filtering the mixture from step (c) to obtain a first precipitate and a first filtrate;
e) dissolving the first precipitate in a second aqueous solution of organic solvent to result in a second mixture, wherein the organic solvent is selected from the group consisting of methanol, ethanol, 1-propanol, isopropanol, and a mixture thereof, and the organic solvent is 70-80 vol. %;
f) inducing crystallization in the second mixture;
g) filtering the mixture from step (f) to obtain a second precipitate and a second filtrate;
h) dissolving the second precipitate in a third aqueous solution of organic solvent to result in a third mixture, wherein the organic solvent is selected from the group consisting of methanol, ethanol, 1-propanol, isopropanol, and a mixture thereof, and the organic solvent is 10-80 vol. %;
i) inducing crystallization in the third mixture; and
j) filtering the mixture from step (i) to obtain a third precipitate and a third filtrate;
whereby the third precipitate is dried to yield the natural sweetener composition.

### Claim 3 (depends on 1)

The low-calorie carbonated drink of claim 1, wherein the natural sweetener composition is in the range of 0.002-0.1% (w/v);
the cola flavor in the range of 0.1-0.5% (w/v);
the phosphoric acid in the range of 0.05-0.2% (v/v);
the citric acid in the range of 0.01-0.03% (w/v);
the carbonated water in the range of 90-99% (v/v); and
the beverage ingredients in the range of 0.1-1% (w/v).

### Claim 4 (depends on 2)

The low-calorie carbonated drink of claim 2, wherein the first filtrate is concentrated into a steviol glycoside mixture.

### Claim 5 (depends on 2)

The low-calorie carbonated drink of claim 2, wherein the step of (i) inducing crystallization in the third mixture comprises adding Rebaudioside D to promote crystallization.

### Claim 6 (depends on 1)

The low-calorie carbonated drink of claim 1, wherein the beverage ingredients include sugar, green tea extract, salt, juniper tincture, ascorbic acid, sodium benzoate, orange red color or fruit flavor or fruit juice selected from the group consisting of orange, apple, lemon, apricot, cherry, and pineapple.

### Claim 7 (depends on 1)

A process for producing the low-calorie carbonated drink of claim 1, comprising:
admixing the natural sweetener composition, cola flavor, phosphoric acid, citric acid, carbonated water, and one or more beverage ingredients; wherein the natural sweetener composition is obtained from Stevia rebaudiana Bertoni plant by a method comprising the following steps:
a) providing an extract of Stevia rebaudiana Bertoni plant;
b) dissolving the extract in a first aqueous solution of organic solvent to result in a first mixture of steviol glycosides, wherein the organic solvent is selected from the group consisting of methanol, ethanol, 1-propanol, isopropanol, and a mixture thereof, and the organic solvent is 75-99 vol. %;
c) inducing crystallization in the first mixture;
d) filtering the mixture from step (c) to obtain a first precipitate and a first filtrate;
e) dissolving the first precipitate in a second aqueous solution of organic solvent to result in a second mixture, wherein the organic solvent is selected from the group consisting of methanol, ethanol, 1-propanol, isopropanol, and a mixture thereof, and the organic solvent is 70-80 vol. %;
f) inducing crystallization in the second mixture;
g) filtering the mixture from step (f) to obtain a second precipitate and a second filtrate;
h) dissolving the second precipitate in a third aqueous solution of organic solvent to result in a third mixture, wherein the organic solvent is selected from the group consisting of methanol, ethanol, 1-propanol, isopropanol, and a mixture thereof, and the organic solvent is 10-80 vol. %;
i) inducing crystallization in the third mixture; and
j) filtering the mixture from step (i) to obtain a third precipitate and a third filtrateo;
whereby the third precipitate is dried to yield the natural sweetener composition;
whereby all components are admixed so as to produce the low-calorie carbonated drink.

### Claim 8 (depends on 7)

The process of claim 7, wherein the beverage ingredients include sugar, green tea extract, salt, juniper tincture, ascorbic acid, sodium benzoate, orange red color or fruit flavor or fruit juice selected from the group consisting of orange, apple, lemon, apricot, cherry, and pineapple.

### Claim 9 (depends on 7)

The process of claim 7, wherein
the natural sweetener composition is in the range of 0.002-0.1% (w/v);
the cola flavor in the range of 0.1-0.5% (w/v);
the phosphoric acid in the range of 0.05-0.2% (v/v);
the citric acid in the range of 0.01-0.03% (w/v);
the carbonated water in the range of 90-99% (v/v); and
the beverage acceptable ingredients in the range of 0.1-1% (w/v).

### Claim 10 (depends on 7)

The process of claim 7, wherein the first filtrate is concentrated into a steviol glycoside mixture.

### Claim 11 (depends on 7)

The process of claim 7, wherein the step of (i) inducing crystallization in the third mixture comprises adding high purity Rebaudioside D to promote crystallization.