# US 8,568,804 B2 · Muscadine compositions with improved anti-oxidant activity

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- Number: US 8,568,804 B2
- Document ID: US8568804B2
- Status: Granted
- Kind: B2
- Application number: 13/056,536
- Filed: 2009-07-31
- Published: 2011-07-21
- Granted: 2013-10-29
- Earliest claimed: 2008-07-31
- Assignees: Shaklee Corporation
- Applicants: Laurel A. Fisher; Teodoro T. Ianiro; William J. Mergens; Nasrin Zamanian
- Inventors: Laurel A. Fisher; Teodoro T. Ianiro; William J. Mergens; Nasrin Zamanian
- Web page: https://patentgrep.com/patent/US8568804B2

## CPC

- A61K 31/7048: having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin {, digitoxin or digoxin}
- A23L 33/105: Plant extracts, their artificial duplicates or their derivatives
- A61K 31/05: Phenols {(cannabinoids A61K31/658)}
- A61K 36/87: Vitaceae or Ampelidaceae (Vine or Grape family), e.g. wine grapes, muscadine or peppervine
- A61P 3/10: for hyperglycaemia, e.g. antidiabetics
- A61P 9/10: for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
- A61P 17/18: Antioxidants, e.g. antiradicals (preparations for protection against sunlight A61Q17/00)
- A61P 25/16: Anti-Parkinson drugs
- A61P 25/28: for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
- A61P 29/00: Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
- A61P 35/00: Antineoplastic agents
- A61P 39/06: Free radical scavengers or antioxidants
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION

## Abstract

Disclosed is a composition for use as an antioxidant, such as for use as an anti-aging supplement. The composition can include a muscadine (Vitis rotundifolia) pomace extract having a polyphenol content of at least 2% and trans-resveratrol from a source other than muscadine with a minimum purity of at least 5%. In an example, the ratio of muscadine pomace extract polyphenols to trans-resveratrol is in the range of 0.1/1 to 10/1 (weight to weight), thereby providing a composition with antioxidant activity. Also disclosed are methods of producing antioxidant compositions. These methods can include combining a muscadine pomace extract having a polyphenol content of at least 2% and resveratrol from a source other than muscadine with a minimum purity of at least 5% at a ratio in the range of 0.1/1 to 10/1 (weight to weight), thereby providing a composition with antioxidant activity.

## Claims

### Claim 1

A composition for use as an antioxidant, comprising
a muscadine (Vitis rotundifolia) pomace solvent extract comprising a liquid bronze muscadine pomace extract combined with a liquid purple muscadine pomace extract to produce a liquid muscadine pomace extract, wherein a) the bronze muscadine pomace extract and the purple muscadine pomace extract are solvent extracts; b) the mixture of bronze muscadine pomace extract and purple muscadine pomace extract promotes solubility of ellagic acid in the muscadine pomace extract; c) the bronze muscadine pomace extract and the purple muscadine pomace extract are filtered and fermented extracts; and d) the muscadine pomace solvent extract has a polyphenol content of at least 2%; and
resveratrol from a source other than muscadine with a minimum purity of at least 5% trans-resveratrol,
wherein a ratio of muscadine pomace extract polyphenols to trans-resveratrol is in the range of 0.1/1 to 10/1 (weight to weight), and wherein the composition has antioxidant activity.

### Claim 2 (depends on 1)

The composition of claim 1, wherein resveratrol from a source other than muscadine comprises at least 98% trans-resveratrol extracted from Japanese Knotweed (Polygonum cuspidatum) root.

### Claim 3 (depends on 1)

The composition of claim 1, wherein the muscadine (Vitis rotundifolia) pomace extract has a total polyphenol content of at least 4% and the ratio of the muscadine pomace extract to resveratrol is about 0.2/1 to 50/1 (weight to weight).

### Claim 4 (depends on 3)

The composition of claim 3 wherein the ratio of the muscadine pomace extract to resveratrol is about 5/1 to 50/1 (weight to weight).

### Claim 5 (depends on 4)

The composition of claim 4 wherein the ratio of the muscadine pomace extract to resveratrol is about 18 to 1 (weight to weight).

### Claim 6 (depends on 1)

The composition of claim 1, further comprising an elderberry extract, a purple carrot extract or a combination thereof.

### Claim 7 (depends on 1)

The composition of claim 1, further comprising an excipient.

### Claim 8 (depends on 7)

The composition of claim 7, wherein the excipient comprises glycerin, sorbitol, colloidal silicon dioxide, a natural flavoring additive, or a combination thereof.

### Claim 9 (depends on 1)

The composition of claim 1, wherein the composition has a total Oxygen Radical Absorbance Capacity (ORAC) of at least 24 μmole Trolox Equivalents per mg polyphenol (μmoleTE/mg polyphenol).

### Claim 10 (depends on 1)

The composition of claim 1, wherein the composition is provided in a non-beverage food, a beverage, liquid or solid dietary supplement or topical ointment.

### Claim 11 (depends on 1)

The composition of claim 1 that reduces or inhibits oxidative stress associated with food consumption.

### Claim 12 (depends on 1)

The composition of claim 1, wherein the solvent is water.

### Claim 13 (depends on 1)

A method of inhibiting oxidative stress, comprising administering to a subject in need thereof a dose of the composition of claim 1 sufficient to reduce or inhibit oxidative stress.

### Claim 14 (depends on 13)

The method of claim 13, wherein the composition is administered 10 to 30 minutes before or after food consumption.

### Claim 15 (depends on 14)

The method of claim 14, wherein the composition is administered 10 minutes before food consumption.

### Claim 16

A composition for use as an antioxidant, comprising
a muscadine (Vitis rotundifolia) pomace solvent extract comprising a mixture of a bronze muscadine pomace extract and a purple muscadine extract, wherein a) the bronze muscadine pomace extract and the purple muscadine pomace extract are liquid solvent extracts that are filtered and fermented extracts, and b) the ratio of bronze muscadine pomace extract to purple muscadine pomace extract ranges from 0.1 to 10 (weight to weight) and has a polyphenol content of at least 2%; and
resveratrol from a source other than muscadine with a minimum purity of at least 5% trans-resveratrol;
wherein a ratio of muscadine pomace extract polyphenols to trans-resveratrol is in the range of 0.1/1 to 10/1 (weight to weight), and wherein the composition has anti-oxidant activity.

### Claim 17 (depends on 16)

The composition of claim 16, wherein the ratio of bronze muscadine pomace extract to purple muscadine pomace extract ranges from 0.3 to 3 (weight to weight).

### Claim 18 (depends on 16)

The composition of claim 16, wherein the muscadine pomace extract comprises 20% to 50% solids in a liquid.

### Claim 19 (depends on 18)

The composition of claim 18, wherein the muscadine pomace extract comprises about 40% solids in a liquid.

### Claim 20 (depends on 16)

The composition of claim 16 wherein the muscadine (Vitis rotundifolia) pomace extract has a total polyphenol content of about 4%.

### Claim 21 (depends on 16)

The composition of claim 16, wherein the solvent is water.

### Claim 22 (depends on 16)

The composition of claim 16, further comprising an elderberry extract, a purple carrot extract or a combination thereof.

### Claim 23 (depends on 16)

The composition of claim 16, further comprising an excipient.

### Claim 24 (depends on 23)

The composition of claim 23, wherein the excipient comprises glycerin, sorbitol, colloidal silicon dioxide, a natural flavoring additive, or a combination thereof.

### Claim 25 (depends on 16)

The composition of claim 16, wherein the composition is provided in a non-beverage food, a beverage, liquid or solid dietary supplement or topical ointment.

### Claim 26 (depends on 16)

A method of inhibiting oxidative stress, comprising administering to a subject in need thereof a dose of the composition of claim 16 sufficient to reduce or inhibit oxidative stress.

### Claim 27 (depends on 26)

The method of claim 26, wherein the composition is administered 10 to 30 minutes before or after food consumption.

### Claim 28 (depends on 27)

The method of claim 27, wherein the composition is administered 10 minutes before food consumption.