# US 8,568,758 B2 · Corn steep liquor as a biostimulant composition > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,568,758 B2 - Document ID: US8568758B2 - Status: Granted - Kind: B2 - Application number: 12/847,706 - Filed: 2010-07-30 - Published: 2012-02-02 - Granted: 2013-10-29 - Earliest claimed: 2010-07-30 - Assignees: Daniels AgroSciences, LLC - Applicants: Ralph S. Daniels - Inventors: Ralph S. Daniels - Web page: https://patentgrep.com/patent/US8568758B2 ## CPC - C12N 1/38: Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound (C12N1/34 takes precedence) - A01N 65/44: Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass - C05F 5/004: {Liquid waste from mechanical processing of material, e.g. wash-water, milling fluid, filtrate} - C12N 1/20: Bacteria; Culture media therefor - Y02A 40/20: Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - C05F: ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE - Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE ## Abstract Aspects of the invention relate to organic biostimulant compositions, including, for example, formulations comprising corn steep liquor (CSL) and water. In certain illustrative embodiments, the organic biostimulant composition further comprises from one to five microbial strains as an inoculant. Another aspect of the invention relates to a method of cultivating plants that comprises the steps of preparing an organic biostimulant composition comprising water and CSL, and applying the organic biostimulant composition through a delivery system to a growth medium supporting plants. ## Claims ### Claim 1 An aqueous organic biostimulant composition consisting of water, oilseed extract, Bacillus subtillis, and corn steep liquor; and wherein the concentration of the corn steep liquor in the aqueous composition is between about 0.10% and about 100% by weight; with optional additives in the composition selected from one or more of: (a) a supplement selected from the group consisting of natural glycerol, humate, fulvate, acetic acid, propionic acid, citric acid, lactic acid, or combinations thereof; and (b) from one to five additional microbial strains as an inoculant; and wherein the aqueous organic biostimulant composition when applied to a growth medium is effective in enhancing the growth of indigenous microorganisms present in the growth medium. ### Claim 2 (depends on 1) The aqueous organic biostimulant composition of claim 1, wherein the concentration of the corn steep liquor is between about 0.10% and 10% by weight, with the remainder of the composition consisting of water. ### Claim 3 (depends on 1) The aqueous organic biostimulant composition of claim 1, wherein the concentration of the corn steep liquor is between about 10% and 90% by weight, with the remainder of the composition consisting of water. ### Claim 4 (depends on 1) The aqueous organic biostimulant composition of claim 1, wherein the concentration of the corn steep liquor is between about 20% and 80% by weight, with the remainder of the composition consisting of water. ### Claim 5 (depends on 1) The aqueous organic biostimulant composition of claim 1, wherein the concentration of the corn steep liquor is between about 40% and 60% by weight, with the remainder of the composition consisting of water. ### Claim 6 (depends on 1) The aqueous organic biostimulant composition of claim 1, further including a supplement selected from the group consisting of natural glycerol, humate, fulvate, acetic acid, propionic acid, citric acid, lactic acid, or combinations thereof. ### Claim 7 (depends on 1) The aqueous organic biostimulant composition of claim 1, further including from one to five microbial strains as an inoculant; and wherein the concentration of the corn steep liquor is between about 0.10% and about 100% by weight. ### Claim 8 (depends on 7) The organic biostimulant composition of claim 7, wherein the number of additional microbial strains in the inoculant is from one to three. ### Claim 9 (depends on 7) The organic biostimulant composition of claim 7, further including a supplement selected from the group consisting of natural glycerol, humate, fulvate, acetic acid, propionic acid, citric acid, lactic acid, or combinations thereof. ### Claim 10 (depends on 7) The organic biostimulant composition of claim 7, wherein the organic biostimulant is effective in enhancing the growth of indigenous microorganisms in a growth medium and the one to five microbial strains in the inoculant. ### Claim 11 A method of cultivating plants, comprising the steps of: preparing an organic biostimulant composition consisting essentially of water, corn steep liquor, oilseed extract, and Bacillus subtillis; and applying the organic biostimulant composition through a delivery system to a growth medium supporting plants. ### Claim 12 (depends on 11) The method of cultivating plants of claim 11, wherein the concentration of the corn steep liquor in the organic biostimulant composition is between about 0.10% and 100% by weight. ### Claim 13 (depends on 11) The method of cultivating plants of claim 11, wherein the organic biostimulant composition further includes a supplement selected from the group consisting of natural glycerol, humate, fulvate, acetic acid, propionic acid, citric acid, lactic acid, or combinations thereof. ### Claim 14 (depends on 11) The method of cultivating plants of claim 11, wherein the organic biostimulant enhances the growth of indigenous microorganisms in the growth medium. ### Claim 15 (depends on 11) The method of cultivating plants of claim 11, wherein the organic biostimulant composition further includes from one to five microbial strains as an inoculant. ### Claim 16 (depends on 15) The method of cultivating plants of claim 15, further including producing at least one economically valuable response as a consequence of applying the biostimulant composition to the growth medium. ### Claim 17 (depends on 16) The method of cultivating plants of claim 16, wherein the organic biostimulant enhances the growth of indigenous microorganisms in the growth medium and the one to five microbial strains in the inoculant.