Methods of culturing cells in a medium comprising transforming growth factor beta 1 and basic fibroblast growth factor
AssigneeTechnion Research & Development Foundation Limited
InventorsMichal Amit, Joseph Itskovitz-Eldor
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Abstract
The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.
Claims25
A method of propagating mammalian pluripotent stem cells in an undifferentiated, pluripotent and proliferative state under culturing conditions devoid of feeder cells, the method comprising culturing the mammalian pluripotent stem cells in a tissue culture medium which comprises transforming growth factor β1 (TGFβ1) and basic fibroblast growth factor (bFGF), to thereby maintain the mammalian pluripotent stem cells in an undifferentiated, pluripotent and proliferative state.
A cell culture comprising mammalian pluripotent stem cells in a culture medium which comprises transforming growth factor β1 (TGFβ1) and basic fibroblast growth factor (bFGF), wherein the cell culture is substantially free of animal- and/or feeder cells contaminants and whereas said mammalian pluripotent stem cells are maintained in an undifferentiated, pluripotent and proliferative state when cultured in said culture medium.
A method of propagating cells in a culture, comprising culturing the cells in a culture medium which comprises transforming growth factor β1 (TGFβ1) and basic fibroblast growth factor (bFGF), wherein said bFGF is provided at a concentration of at least 2 ng/ml, and wherein said TGFβ1 is provided at a concentration range of 0.06-0.24 ng/ml, there propagating the cells in the culture.
A cell culture comprising cells and a culture medium, wherein said culture medium comprises transforming growth factor β1 (TGFβ1) and basic fibroblast growth factor (bFGF), wherein said bFGF is provided at a concentration of at least 2 ng/ml, and wherein said TGFβ1 is provided at a concentration range of 0.06-0.24 ng/ml.
Dependencies are inferred from the claim text.