Controllable binding and dissociation of chemical entities and electrode devices therefore
AssigneeTechnion Research & Development Foundation Limited
InventorsUri Sivan, Elad Brod
Life of this patent
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Abstract
Disclosed are methods for controlling molecular interactions where at least one member of a pair of chemical entities having a pH-dependent affinity is immobilized on the surface of an electrode. By changing the potential difference between the electrode and a counter electrode, the affinity of the pair of chemical entities is changed, changing the tendency of the pair to bind or to dissociate. Disclosed are also devices useful for implementing the methods of the present invention.
Claims24
A method of changing the affinity of a pair of chemical entities having a pH-dependent affinity, wherein the pair of chemical entities constitutes an affinity pair being a pair of two molecules that are capable of mutually bind non-covalently with high specificity and dissociate, the method comprising:
- a) immobilizing a first member of said pair of chemical entities having a pH-dependent affinity to a surface of an electrode;
- b) immersing said first member of said pair of chemical entities in an electrolysable environment so that said electrode is in electrical contact with said environment where a second member of said pair of chemical entities is found in said environment;
- c) making electrical contact between a counter electrode and said environment;
- and
- d) changing a potential between said electrode and said counter electrode so as to substantially change electrolysis of said environment thereby changing said affinity of said chemical entities so as to bind or dissociate reversibly by substantially changing a concentration of electrolysis products in said environment in proximity of said surface of said electrode.
A method of changing the affinity of a pair of chemical entities having a pH-dependent affinity, comprising:
- a) immobilizing a first member of said pair to a surface of an electrode and immobilizing a second member of said pair to said surface so that said first member and said second member of said pair are within binding distance;
- b) immersing said first member and said second member of said pair in an electrolysable environment so that said electrode is in electrical contact with said environment;
- c) making electrical contact between a counter electrode and said environment;
- and
- d) changing a potential between said electrode and said counter electrode so as to substantially change electrolysis of said environment thereby changing said affinity of said first member and said second member reversibly by substantially changing a concentration of electrolysis products in said environment in proximity of said surface of said electrode.
A method of changing the affinity of a DNA-histone affinity pair, comprising:
- a) immobilizing said DNA to a surface of an electrode and immobilizing said histone to said surface so that said DNA and said histone are within binding distance;
- b) immersing said DNA and said histone in an electrolysable environment so that said electrode is in electrical contact with said environment;
- c) making electrical contact between a counter electrode and said environment;
- and
- d) changing a potential between said electrode and said counter electrode so as to substantially change electrolysis of said environment thereby changing said affinity of said pair reversibly by substantially changing a concentration of electrolysis products in said environment in proximity of said surface of said electrode.
A method of changing the affinity of a pair of chemical entities having a pH-dependent affinity, wherein the chemical entities constitute an affinity pair having each member thereof selected from the group consisting of a peptide, a polypeptide and a protein, the method comprising:
- a) immobilizing a first member of said pair of chemical entities having a pH-dependent affinity to a surface of an electrode;
- b) immersing said first member of said pair of chemical entities in an electrolysable environment so that said electrode is in electrical contact with said environment where a second member of said pair of chemical entities is found in said environment;
- c) making electrical contact between a counter electrode and said environment;
- and
- d) changing a potential between said electrode and said counter electrode so as to substantially change electrolysis of said environment thereby changing said affinity of said chemical entities so as to bind or dissociate reversibly by substantially changing a concentration of electrolysis products in said environment in proximity of said surface of said electrode.
Dependencies are inferred from the claim text.