Electrochemical affinity biosensor system and methods
AssigneeRoche Diagnostics Operations, Inc.
InventorsEric R. Diebold, Mitali Ghoshal, David Z. Deng, Jane Shiow-Chuan Tsai
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Abstract
The present invention provides novel osmium-based electrochemical species for the detection of wide variety of analytes using immunological techniques. The present invention also provides diagnostic kits and test sensors supporting electrode structures that can be used with the osmium-based electrochemical species. The test sensor can be fabricated to support interdigitated arrays of electrodes that have been designed to provide amplification of the electrical signal amplification desired to analyze analytes that may be present at low concentrations.
Claims10
A compound of the formula:
- wherein, R and R1 are the same or different and each can be selected from:
- 2,2′-bipyridyl, 4,4′-disubstituted-2,2′-bipyridyl, 5-5′-disubstituted-2,2′-bipyridyl, 1,10-phenanthrolinyl, 4,7-disubstituted-1,10-phenanthrolinyl, 5,6-disubstituted-1,10-phenanthrolinyl, or N,N′-dimethyl 2,2′-biimidazole wherein each substituent is a methyl, ethyl, or phenyl group, and where the R and R′ groups are coordinated to Os through their nitrogen atoms;
- R3 is H, CH3 or C2H5;
- L is (CH2)iQ, wherein i is an integer 1-10 and Q is O or NR3;
- B is a group comprising a ligand capable of binding to a specific analyte binding partner;
- Z is chlorine or bromine;
- X is a counter ion;
- y is selected to provide a neutral salt;
- and m is 1 or 2.
A compound of the formula:
- wherein, R and R1 are the same or different and each can be selected from:
- 2,2′-bipyridyl, 4,4′-disubstituted-2,2′-bipyridyl, 5,5′-disubstituted-2,2′-bipyridyl, 1,10-phenanthrolinyl, 4,7-disubstituted-1,10-phenanthrolinyl, 5,6-disubstituted-1,10-phenanthrolinyl, or N,N′-dimethyl 2, 2′-biimidazole wherein each substituent is a methyl, ethyl, or phenyl group, and where the R and R1 groups are coordinated to Os through their nitrogen atoms, R3 is H, CH3 or C2H5;
- L is (CH2)iQ, wherein i is an integer 1-10 and Q is O or NR3—;
- A is —(CH2)j—NR3, —(CH2)j—SH, or an activated ester wherein j is an integer between 1-5;
- Z is chlorine or bromine;
- X is a counter ion;
- y is selected to provide a neutral salt;
- and m is 1 or 2.
Dependencies are inferred from the claim text.