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US 8,557,974 B2Granted
Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria
AssigneeMolecular Detection, Inc.
InventorYosef Paitan
Life of this patent
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Abstract
The present invention relates generally to detection of antibiotic-resistant bacteria in a sample. In particular, the invention provides methods, compositions and kits for detecting and analyzing methicillin-resistant Staphylococcus aureus (MRSA) and other methicillin-resistant bacteria in a sample.
Claims3
A kit that comprises:
- (a) a first set of primers complementary to at least a portion of a mecA polynucleotide sequence;
- (b) a second set of primers complementary to at least a portion of a bridging region;
- (c) a third set of primers complementary to at least a portion of an MSSA-orfX polynucleotide sequence:
- and
- (d) at least one member selected from:
- a DNA polymerase enzyme, (dNTPs, magnesium and a stabilizer.
3 more elements
A kit for detecting an antibiotic-resistant bacterial strain in a sample, wherein said kit comprises:
- (a) a first set of primers, wherein said first set of primers are capable of producing a first amplification product from at least a portion of a gene, wherein said gene confers antibiotic-resistance;
- (b) a second set of primers, wherein said second set of primers are capable of producing a second amplification product from at least a portion of a bridging region;
- (c) a third set of primers, wherein said third set of primers are capable of producing a third amplification product from at least a portion of a bacterial strain-specific polynucleotide sequence.
Dependencies are inferred from the claim text.