US 8,557,972 B2Granted

Compositions and methods for transfection of RNA and controlled stabilization of transfected RNA

AssigneeUniversity of Washington through its Center for Commercialization

InventorsThomas A. Reh, Olivia M. Bermingham-McDonogh, Toshinori Hayashi

Life of this patent

Estimating the term…

The present invention provides reagents and methods for RNA transfection and protein expression.

Dependent claims

A recombinant nucleic acid, comprising

(a) an RNA molecule;
and
(b) a first recombinant RNA comprising two or more copies of a 3′ untranslated region (UTR) of an RNA virus selected from the group consisting of Venezuelan equine encephalitis virus (VEEV) and Sinbis virus;
wherein the first recombinant RNA is coupled to the 3′ end of the RNA molecule.

1 more element

Dependencies are inferred from the claim text.

C12N 15/67
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression: general methods for enhancing…
C12N 5/062
Cells of the nervous system: sensory transducers, e.g. photoreceptors; Sensory neurons, e.g. for hearing, taste, smell, pH, touch, temperature, pain
C12N 2501/60
Active agents used in cell culture processes, e.g. differentation: transcription factors
C12N 2510/00
Genetically modified cells

From the US filing history. Foreign family members aren’t listed.

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