Homodimer of insulinotropic peptide analogues and method for preparation thereof and use thereof

A method for preparing a homodimer of insulinotropic peptide analogues, comprising:

• conjugating two identical insulinotropic peptide analogues at the C-terminal Cys residue via disulfide bond oxidated from free sulfhydryl thereon,
• wherein said two identical insulinotropic peptide analogues are Exendin-4 derivatives with the amino acid sequence of SEQ ID NO:2:
• His Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gln Met Glu Glu Glu Ala Val Arg Leu Phe Ile Glu Trp Leu Lys Asn Xaa Cys-N,
• wherein N is —OH or —NH2, and
• wherein Xaa is:
• A:
• (GlyGlySer) n, or (GlyGlyPro) n, wherein n is an integer between 1-5;
• B:
• (Gly) n, or (Gly) n Ser, wherein n is an integer between 4-20;
• or
• C:
• any combination of A or B.

A drug for the treatment of metabolism syndromes related to diabetes or hyperglycemia, comprising a homodimer of insulinotropic peptide analogues prepared by conjugating two identical insulinotropic peptide analogues at their C-terminal Cys residues,

• wherein the insulinotropic peptide analogues are Exendin-4 derivatives with the amino acid sequence of SEQ ID NO:2:
• His Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gln Met Glu Glu Glu Ala Val Arg Leu Phe Ile Glu Trp Leu Lys Asn Xaa Cys-N,
• wherein N is —OH or —NH2, and
• wherein Xaa is:
• A:
• (GlyGlySer) n, or (GlyGlyPro) n, wherein n is an integer between 1-5;
• B:
• (Gly) n, or (Gly) n Ser, wherein n is an integer between 4-20;
• or
• C:
• any combination of A or B.