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US 8,557,768 B1Granted
Human nerve growth factor by recombinant technology
AssigneeGenentech, Inc.
InventorsAlane M. Gray, Axel Ullrich
Life of this patent
Estimating the term…
Abstract
The β-subunit of human nerve growth factor (βNGF) is prepared in essentially pure form in commercially useful quantities using recombinant DNA technology. The nucleotide sequence and vectors encoding human βNGF and host cells transformed with the vectors are also provided. The βNGF which the invention provides is useful, for example, in treating nerve damage.
Claims8
A composition comprising a βNGF polypeptide which comprises a human βNGF amino acid sequence selected from the group consisting of:
- Ser Ser Ser His Pro Ile Phe His Arg Gly Glu Phe Ser Val Cys
- Asp Ser Val Ser Val Tip Val Gly Asp Lys Thr Thr Ala Thr Asp
- Ile Lys Gly Lys Glu Val Met Val Leu Gly Glu Val Asn Ile Asn
- Asn Ser Val Phe Lys Gln Tyr Phe Phe Glu Thr Lys Cys Arg Asp
- Pro Asn Pro Val Asp Ser Gly Cys Arg Gly Ile Asp Ser Lys His
- Trp Asn Ser Tyr Cys Thr Thr Thr His Thr Phe Val Lys Ala Leu
- Thr Met Asp Gly Lys Gln Ala Ala Trp Arg Phe Ile Arg Ile Asp
- Thr Ala Cys Val Cys Val Leu Ser Arg Lys Ala Val Arg,
- Ser Ser Ser His Pro Ile Phe His Arg Gly Glu Phe Ser Val Cys
- Asp Ser Val Ser Val Trp Val Gly Asp Lys Thr Thr Ala Thr Asp
- Ile Lys Gly Lys Glu Val Met Val Leu Gly Glu Val Asn Ile Asn
- Asn Ser Val Phe Lys Gln Tyr Phe Phe Glu Thr Lys Cys Arg Asp
- Pro Asn Pro Val Asp Ser Gly Cys Arg Gly Ile Asp Ser Lys His
- Trp Asn Ser Tyr Cys Thr Thr Thr His Thr Phe Val Lys Ala Leu
- Thr Met Asp Gly Lys Gln Ala Ala Trp Arg Phe Ile Arg Ile Asp
- Thr Ala Cys Val Cys Val Leu Ser Arg Lys Ala Val Arg Arg Ala, and
- Met Ser Ser Ser His Pro Ile Phe His Arg Gly Glu Phe Ser Val Cys
- Asp Ser Val Ser Val Trp Val Gly Asp Lys Thr Thr Ala Thr Asp
- Ile Lys Gly Lys Glu Val Met Val Leu Gly Glu Val Asn Ile Asn
- Asn Ser Val Phe Lys Gln Tyr Phe Phe Glu Thr Lys Cys Arg Asp
- Pro Asn Pro Val Asp Ser Gly Cys Arg Gly Ile Asp Ser Lys His
- Trp Asn Ser Tyr Cys Thr Thr Thr His Thr Phe Val Lys Ala Leu
- Thr Met Asp Gly Lys Gln Ala Ala Trp Arg Phe Ile Arg Ile Asp
- Thr Ala Cys Val Cys Val Leu Ser Arg Lys Ala Val Arg,
- and a carrier, wherein said βNGF polypeptide is obtained by a process comprising:
- expressing said βNGF polypeptide in a mammalian host cell;
- and
- recovering said βNGF polypeptide,
- wherein said composition is free of other polypeptides of human origin.
26 more elements
A composition comprising a βNGF polypeptide, wherein said βNGF polypeptide consists essentially of a human βNGF amino acid sequence selected from the group consisting of:
- Ser Ser Ser His Pro Ile Phe His Arg Gly Glu Phe Ser Val Cys
- Asp Ser Val Ser Val Trp Val Gly Asp Lys Thr Thr Ala Thr Asp
- Ile Lys Gly Lys Glu Val Met Val Leu Gly Glu Val Asn Ile Asn
- Asn Ser Val Phe Lys Gln Tyr Phe Phe Glu Thr Lys Cys Arg Asp
- Pro Asn Pro Val Asp Ser Gly Cys Arg Gly Ile Asp Ser Lys His
- Trp Asn Ser Tyr Cys Thr Thr Thr His Thr Phe Val Lys Ala Leu
- Thr Met Asp Gly Lys Gln Ala Ala Trp Arg Phe Ile Arg Ile Asp
- Thr Ala Cys Val Cys Val Leu Ser Arg Lys Ala Val Arg,
- Ser Ser Ser His Pro Ile Phe His Arg Gly Glu Phe Ser Val Cys
- Asp Ser Val Ser Val Trp Val Gly Asp Lys Thr Thr Ala Thr Asp
- Ile Lys Gly Lys Glu Val Met Val Leu Gly Glu Val Asn Ile Asn
- Asn Ser Val Phe Lys Gln Tyr Phe Phe Glu Thr Lys Cys Arg Asp
- Pro Asn Pro Val Asp Ser Gly Cys Arg Gly Ile Asp Ser Lys His
- Trp Asn Ser Tyr Cys Thr Thr Thr His Thr Phe Val Lys Ala Leu
- Thr Met Asp Gly Lys Gln Ala Ala Trp Arg Phe Ile Arg Ile Asp
- Thr Ala Cys Val Cys Val Leu Ser Arg Lys Ala Val Arg Arg Ala, and
- Met Ser Ser Ser His Pro Ile Phe His Arg Gly Glu Phe Ser Val Cys
- Asp Ser Val Ser Val Trp Val Gly Asp Lys Thr Thr Ala Thr Asp
- Ile Lys Gly Lys Glu Val Met Val Leu Gly Glu Val Asn Ile Asn
- Asn Ser Val Phe Lys Gln Tyr Phe Phe Glu Thr Lys Cys Arg Asp
- Pro Asn Pro Val Asp Ser Gly Cys Arg Gly Ile Asp Ser Lys His
- Trp Asn Ser Tyr Cys Thr Thr Thr His Thr Phe Val Lys Ala Leu
- Thr Met Asp Gly Lys Gln Ala Ala Trp Arg Phe Ile Arg Ile Asp
- Thr Ala Cys Val Cys Val Leu Ser Arg Lys Ala Val Arg,
- and a carrier, wherein said β-NGF polypeptide is obtained by a process comprising:
- expressing said βNGF polypeptide in a mammalian cell;
- and
- recovering said βNGF polypeptide,
- wherein said composition is free of other polypeptides of human origin.
26 more elements
Dependencies are inferred from the claim text.