Method for immobilization, physiologically active substance-immobilized carrier, carrier for immobilization, carrier, and process for producing carrier

A physiologically-active-substance-immobilized carrier prepared by immobilizing a physiologically active substance on a solid phase carrier by an immobilization method comprising:

• bringing the solid phase carrier into contact with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I);
• and
• carrying out a treatment for binding of the physiologically active substance to the acid anhydride functional group while maintaining the solid phase carrier after the contact at a temperature within the range of 0° C. to 60° C.:
• wherein, in Formula (I), R1 represents a trivalent linear or branched aliphatic group or aromatic group;
• R2 represents a substituted or unsubstituted C1 to C20 alkylene group, a substituted or unsubstituted C1 to C20 arylene group, a substituted or unsubstituted C1 to C20 arylalkylene group or a substituted or unsubstituted C1 to C20 alkylarylene group;
• R3 represents a hydrogen atom or a C1 to C10 alkyl group;
• X represents an alkoxy group (—OR4), a halogen atom or an acyloxy group (−OOCR5), wherein R4 and R5 each independently represents a hydrogen atom or a C1 to C10 alkyl group;
• n represents 1, 2 or 3;
• and m represents an integer of (3−n).

A carrier for immobilization obtained by bringing a solid phase carrier into contact with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I), in at least one aromatic hydrocarbon based solvent selected from the group consisting of benzene, xylene and toluene, and used for preparing a physiologically-active-substance-immobilized carrier, the preparation of the physiologically-active-substance-immobilized carrier including carrying out a treatment for binding of a physiologically active substance to the acid anhydride functional group while maintaining the carrier for immobilization at a temperature within the range of 0° C. to 60° C.:

• wherein, in Formula (I), R1 represents a trivalent linear or branched aliphatic group or aromatic group;
• R2 represents a substituted or unsubstituted C1 to C20 alkylene group, a substituted or unsubstituted C1 to C20 arylene group, a substituted or unsubstituted C1 to C20 arylalkylene group or a substituted or unsubstituted C1 to C20 alkylarylene group;
• R3 represents a hydrogen atom or a C1 to C10 alkyl group;
• X represents an alkoxy group (—OR4), a halogen atom or an acyloxy group (—OOCR5), wherein R4 and R5 each independently represents a hydrogen atom or a C1 to C10 alkyl group;
• n represents 1, 2 or 3;
• and m represents an integer of (3−n).