Display library process

A method of identifying members of a bacteriophage library that have a desired binding property, the method comprising:

• (a) providing a bacteriophage library that comprises a plurality of bacteriophage members;
• (b) selecting a subset of the bacteriophage members;
• (c) infecting host cells with the members of the subset;
• (d) producing replicate members of the subset from the infected cells in the presence of a target immobilized to a support, thereby forming replicate members immobilized to the support via binding to the target without first purifying or harvesting the replicate members, wherein the producing is completed in less than 2 hours;
• and
• (e) selecting a subset of the amplified members, thereby identifying the desired members of the bacteriophage library.

A method of selecting a nucleic acid that encodes a binding protein from a library of display phage, the method comprising:

• a) providing a library of phage that each have a heterologous protein component that is diverse among the phage of the plurality, physically attached to the phage, and accessible;
• b) contacting phage of the library to a target;
• c) performing one or more cycles of:
• i) forming phage immobilized to a support comprising binding a phage to the target by its heterologous protein component and immobilizing the target to a support,
• ii) separating phage that do not bind to the target from the phage immobilized to the support via binding to the target,
• iii) contacting phage from the phage immobilized to the support with host cells so that the host cells are infected by the phage from the phage immobilized to the support, and
• iv) producing phage from the infected cells in the presence of the target, the produced phage being replicates of phage that bind to the target, wherein the replicates of phage are not purified or harvested before binding to the target, wherein the producing is completed in less than 2 hours, and
• d) recovering the nucleic acid encoding the heterologous protein component of one or more produced phage that bind to the target, thereby selecting a nucleic acid that encodes a binding protein for the target.