Method for measuring blood sample and apparatus thereof

An apparatus for measuring a blood sample in a container sealed by a cap, comprising:

• an aspiration pipe for piercing the cap of the container and aspirating the blood sample in the sealed container, wherein the aspiration pipe comprises a first flow channel extending in a longitudinal direction and an aspiration opening formed near the tip;
• an aspiration pipe moving mechanism for moving the aspiration pipe to an outside position of the sealed container, a first position where the aspiration opening is located above the blood sample in the container, and a second position where the aspiration opening is located in the blood sample in the container;
• a fluid mechanism comprising a second flow channel released to atmospheric air, a diluted solution accommodating part for accommodating a diluted solution, a third flow channel capable of connecting the aspiration pipe to the diluted solution accommodating part, a first pump capable of supplying the diluted solution accommodated in the diluted solution accommodating part to the aspiration pipe via the third flow channel, and a second pump configured for aspirating and discharging the blood sample in the sealed container via the third flow channel, and for selectively communicating the first and the second flow channels with the first flow channel of the aspiration pipe;
• a measurement sample preparing container for mixing the blood sample with a reagent to prepare a measurement sample;
• a measuring part for measuring an analysis sample prepared in the measurement sample preparing container, wherein the measuring part comprises:
• a first detector configured to measure a number of red blood cells in the analysis sample;
• a second detector configured to measure hemoglobin in the analysis sample;
• and
• a third detector configured to measure a number of white blood cells in the analysis sample;
• and
• a controller configured for controlling the aspiration pipe moving mechanism and the fluid mechanism to perform operations comprising:
• moving the aspiration pipe such that the aspiration opening is moved to the first position from the outside position;
• releasing the inside of the sealed container to atmospheric air via the second flow channel;
• moving the aspiration pipe such that the aspiration opening is moved to the outside position from the first position;
• supplying the diluted solution into the aspiration pipe via the third flow channel by the first pump to fill the first flow channel with the diluted solution;
• forming an air gap in the aspiration opening side of the filled first flow channel by the aspiration of the second pump;
• moving the aspiration pipe so that the aspiration opening is moved to the second position from the outside position;
• aspirating the blood sample in the sealed container by the second pump;
• moving the aspiration pipe such that the aspiration opening is moved to a measurement sample preparing container facing position from the second position;
• and
• discharging the aspirated blood sample into the measurement sample preparing container from the aspiration opening of the aspiration pipe.