Cellulose degradable yeast and method for production thereof
AssigneesNational University Corporation Kobe University, Kansai Chemical Engineering Co., Ltd., Bio-Energy Corporation
InventorsAkihiko Kondo, Ryosuke Yamada, Hideo Noda
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Abstract
The present invention provides a method for producing a cellulose degradable yeast, comprising the step of co-introducing genes coding for at least two cellulose-degrading enzymes into a yeast host via integration with a yeast δ sequence. According to the invention, a yeast having an improved cellulose degradation ability are provided.
Claims20
A method for producing a cellulose degradable yeast, comprising the step of:
- co-introducing genes coding for at least two cellulose-degrading enzymes into a yeast host via integration with a yeast δ sequence, wherein the at least two cellulose-degrading enzymes are a combination of enzymes comprising (A) endoglucanase and (B) β-glucosidase, and wherein the ratio for genes of (A)/(B) is 2 or greater.
A method for producing a cellulose degradable yeast, comprising the step of:
- co-introducing genes coding for at least two cellulose-degrading enzymes into a yeast host via integration with a yeast δ sequence, wherein the at least two cellulose-degrading enzymes are a combination of enzymes comprising (A) endoglucanase and (B) cellobiohydrolase, and wherein the ratio for genes of (A)/(B) is greater than 1.
A method for producing a cellulose degradable yeast, comprising the step of:
- co-introducing genes coding for at least three cellulose-degrading enzymes into a yeast host via integration with a yeast δ sequence, wherein the at least three cellulose-degrading enzymes are a combination of enzymes comprising (A) endoglucanase, (B) β-glucosidase, and (C) cellobiohydrolase, and wherein the ratio for genes of (A)/(B) is 2 or greater and the ratio for genes of (A)/(C) is greater than 1.
A method for producing a cellulose degradable yeast, comprising the step of:
- co-introducing genes coding for at least two cellulose-degrading enzymes into a yeast host via cocktail δ integration with at least two vectors containing the respective cellulose-degrading enzymes between a pair of yeast δ sequences, wherein the at least two vectors have an identical yeast selectable marker, wherein the at least two cellulose-degrading enzymes are selected from a group consisting of endoglucanase, cellobiohydrolase, and β-glucosidase.
Dependencies are inferred from the claim text.