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AssigneeRepublic of Korea, Rural Development Administration
InventorsSun Hwa Ha, Ju Kon Kim, Ha Rin Jung, Jung Bong Kim, Young Mi Kim, Seok-Cheol Suh, Liang Ying Shi, Soon Jong Kweon, Dong Hern Kim
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The present invention relates to fusion polynucleotides for biosynthesis of beta-carotene and a method for producing beta-carotene using the same. More particularly, it relates to fusion polynucleotides encoding phytoene synthase, connective sequences of FMDV-derived 2A sequence or internal ribosome entry site (IRES) and carotene desaturase, and a method for producing beta-carotenes using the same. Fusion polynucleotides and the recombinant vector using the same of the present invention have the effect of expression both phytoene synthase gene and carotene desaturase gene stably within cell transformants. Accordingly, fusion polynucleotides of the present invention can be used to regulate the biosynthetic metabolism of plant producing beta-carotene. Furthermore, it can be applied to effectively increase the content of beta-carotene, a useful metabolite.
A recombinant vector comprising a promoter and a fusion polynucleotide for the biosynthesis of beta-carotene comprising the nucleotide sequence as set forth in SEQ ID NO:1 having the phytoene synthase gene of pepper, FMDV-derived 2A sequence optimized in rice plant, and the carotene desaturase gene of bacteria, wherein the recombinant vector is a pGIb-PIC vector or a pGIb-PAC vector.
A recombinant vector comprising a promoter and a fusion polynucleotide for the biosynthesis of beta-carotene comprising the nucleotide sequence as set forth in SEQ ID NO:42 having the phytoene synthase gene of pepper, the internal ribosome entry site (IRES) gene, and the carotene desaturase gene of bacteria, wherein the recombinant vector is a pGIb-PIC vector or a pGIb-PAC vector.
Dependencies are inferred from the claim text.