US 8,557,577 B2 · Solid phase cell isolation and/or enrichment method

Abstract

The present invention concerns a solid phase method for isolating and/or enriching predetermined cells from a sample. Such methods are used e.g. to isolate and enrich predetermined cells like fetal cells from a sample of maternal peripheral blood, tumor cells from a sample of body fluid or stem cells from a fluid or fluidized sample of body tissue or body fluid. The solid phase isolation method of the present invention is used for isolating predetermined cells from a sample containing such predetermined cells by binding the predetermined cells to a solid surface. According to the invention the sample is contacted with the solid surface and then removed from the solid surface, wherein the sample or a washing buffer contains a polyol during or after contacting the sample with the solid surface.

Claims19

Dependent claims

A solid phase isolation method for isolating predetermined target cells from a sample containing the predetermined target cells and non-target cells comprising:

(a) contacting the sample with the solid surface to bind the predetermined target cells to the solid surface, and
(b) washing the contacted sample from (a) with a washing buffer which removes non-bound non-target cells from the solid surface thereby isolating said predetermined target cells on the solid surface wherein the washing buffer comprises at least one polyol selected from sorbitol, trehalose, sucrose, mannitol, fructose, malitol, lacitol and xylitol, wherein the sample contains the polyol at least during one of (a) contacting the sample with the solid surface or (b) washing.

Dependencies are inferred from the claim text.

Solid phase cell isolation and/or enrichment method

Life of this patent

Solid phase cell isolation and/or enrichment method

Life of this patent

Abstract

Claims19