Compositions and methods of producing enterokinase in yeast
AssigneeAllergan, Inc.
InventorsIan Fotheringham, Peter Sheffield
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Abstract
The present specification disclose polynucleotide molecules encoding an enterokinase, yeast expression constructs including a yeast expression vector and a polynucleotide molecules encoding an enterokinase, yeast cells comprising such a yeast expression construct, methods of producing enterokinase using such yeast cells, and method of cleaving or preparing a recombinant polypeptide using an enterokinase produced by such methods.
Claims14
An isolated polynucleotide molecule comprising the nucleotide sequence of SEQ ID NO:
- 4 or SEQ ID NO:
- 6.
A yeast expression construct comprising a yeast expression vector operably-linked to a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
- 4.
A yeast cell transformed with a yeast expression construct comprising a polynucleotide having the nucleotide sequence of SEQ ID NO:
- 4 or SEQ ID NO:
- 6.
A method of producing an enterokinase, the method comprising the step of culturing a yeast cell transformed with a yeast expression construct comprising a polynucleotide haying the nucleotide sequence of SEQ ID NO:
- 4 or SEQ ID NO:
- 6 to thereby produce an enterokinase encoded by the nucleotide sequence of SEQ ID NO:
- 4 or SEQ ID NO:
- 6.
Dependencies are inferred from the claim text.