Mutation of DNA polymerases from archaeobacteria
AssigneeThe University of Newcastle
InventorsBernard Connolly, Mark Fogg, Laurence H Pearl
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Abstract
The present invention relates to a variant archaeal DNA polymerase having a modified amino acid sequence of a wild-type amino acid sequence, the modified sequence being in the amino-terminal amino acids that comprise a uracil-binding pocket in the wild-type polymerase whereby the variant polymerase has reduced affinity for uracil than the wild-type polymerase. Such variant polymerases may be usefully employed in biological assay systems such as the polymerase chain reaction.
Claims6
A variant archaeal family B DNA polymerase comprising the amino acid sequence of SEQ ID NO:2 with a substitution at position V93, the substitution being in the amino-terminal amino acids that comprise a uracil-binding pocket in a wild-type family B DNA polymerase amino acid sequence whereby the variant polymerase has reduced affinity for uracil as compared to the wild-type polymerase.
Dependencies are inferred from the claim text.