Nuclease reduction

A method for reducing initial nuclease activity and maintaining subtilisin activity in a subtilisin solution comprising:

• a) adjusting the pH of the subtilisin solution to a pH in the range of from pH 8.5 to pH 10.5,
• b) adding a polyol in an amount of 10% (v/v) to 60% (v/v);
• c) adjusting the temperature of the subtilisin solution to a temperature in the range of from 50° C. to 80° C.;
• and
• (d) incubating the subtilisin- and nuclease-comprising solution for a period of up to 10 minutes,
• wherein nuclease activity is reduced to at least less than 5% of the initial value and the subtilisin activity is maintained at more than 70% of the initial value.

A method for reducing initial nuclease activity and maintaining subtilisin activity in a subtilisin solution comprising:

• a) providing an ultrafiltrated subtilisin fermentation broth solution at a pH of pH 8.5 to pH 10.5;
• b) adding a polyol in an amount of 10% (v/v) to 60% (v/v);
• c) adjusting the temperature of the subtilisin solution to a temperature in the range of from 50° C. to 80° C.;
• and
• (d) incubating the subtilisin- and nuclease-comprising solution for a period of up to 10 minutes,
• wherein nuclease activity is reduced to at least less than 5% of the initial value and the subtilisin activity is maintained at more than 70% of the initial value.

A method for reducing nuclease activity in a subtilisin solution comprising:

• a) providing an ultrafiltrated subtilisin fermentation broth solution at a pH of pH 8.5 to pH 10.5;
• b) adding a polyol in an amount of 10% (v/v) to 60% (v/v);
• c) adjusting the temperature of the subtilisin solution to a temperature in the range of from 50° C. to 80° C.;
• and
• (d) incubating the subtilisin- and nuclease-comprising solution for a period of up to 10 minutes,
• wherein the nuclease activity is reduced to less than 5% of the initial value and the subtilisin activity is maintained at more than 80% of the initial value.