Pyripyropene a biosynthetic gene
AssigneeMeija Seika Pharma Co., Ltd.
InventorsHiroyuki Anzai, Kentaro Yamamoto, Mariko Tsuchida, Kazuhiko Oyama, Masaaki Mitomi
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Abstract
An isolated novel polynucleotide comprising a nucleotide sequence encoding at least one polypeptide involved in biosynthesis of pyripyropene A, a recombinant vector comprising the polynucleotide and a transformant comprising the polynucleotide are disclosed. By the present invention, a pyripyropene A biosynthetic gene useful for production of a novel pyripyropene analog, improvement of productivity of a pyripyropene A-producing bacterium, production of an insecticidal agent for microorganisms, creation of a plant resistant to insect pests or the like are provided.
Claims19
A recombinant vector comprising one or more of an isolated polynucleotide which is
- (a) a polynucleotide having a nucleotide sequence of SEQ ID NO:266,
- (b) a polynucleotide having a nucleotide sequence which is capable of hybridizing with the nucleotide sequence of SEQ ID NO:266 under stringent conditions comprising washing in a solution comprising 30 mM trisodium citrate, 300 mM sodium chloride and 0.5% SDS at 60° C. for 20 minutes,
- (c) a polynucleotide having a polynucleotide sequence encoding at least one amino acid sequence selected from SEQ ID NOs:267 to 274 or an amino acid sequence selected from SEQ ID NOs:267 to 274 having 1 to 40 substituted, deleted, added or inserted residues and having the same enzymatic activity as SEQ ID NOs:267 to 274, respectively, or
- (d) a polynucleotide having at least one nucleotide sequence selected from the nucleotide sequence of any of (1) or (2) below:
- (1) a nucleotide sequence of any of (i) to (viii) below:
- (i) a nucleotide sequence from positions 3342 to 5158 of the nucleotide sequence shown in SEQ ID NO:266,
- (ii) a nucleotide sequence from positions 5382 to 12777 of the nucleotide sequence shown in SEQ ID NO:266,
- (iii) a nucleotide sequence from positions 13266 to 15144 of the nucleotide sequence shown in SEQ ID NO:266,
- (iv) a nucleotide sequence from positions 16220 to 18018 of the nucleotide sequence shown in SEQ ID NO:266,
- (v) a nucleotide sequence from positions 18506 to 19296 of the nucleotide sequence shown in SEQ ID NO:266,
- (vi) a nucleotide sequence from positions 19779 to 21389 of the nucleotide sequence shown in SEQ ID NO:266,
- (vii) a nucleotide sequence from positions 21793 to 22877 of the nucleotide sequence shown in SEQ ID NO:266,
- (viii) a nucleotide sequence from positions 23205 to 24773 of the nucleotide sequence shown in SEQ ID NO:266;
- (2) a nucleotide sequence which is capable of hybridizing with a nucleotide sequence of (1) under stringent conditions comprising washing in a solution comprising 30 mM trisodium citrate, 300 mM sodium chloride and 0.5% SDS at 60° C. for 20 minutes.
Dependencies are inferred from the claim text.