Phosphotriesterase from agrobacterium radiobacter P230
AssigneeCommonwealth Scientific and Industrial Research Organisation
InventorsIrene Horne, Tara Sutherland, Rebecca Harcourt, Robyn Russell, John Oakeshott
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Abstract
The present invention provides enzymes capable of hydrolysing organophosphate (OP) molecules. In particular, the invention provides a phosphotriesterase enzyme identified from an Agrobacterium radiobacter strain isolated from soil that hydrolyses OP pesticides, and the gene encoding that enzyme. The invention also provides mutants of the identified phosphotriesterase enzyme which have altered substrate specificity. The use of these enzymes in bioremediation strategies is also provided.
Claims21
A host cell comprising a recombinant polynucleotide which encodes a polypeptide comprising amino acids whose sequence is at least 95% identical to the sequence set forth as SEQ ID NO:2 or a composition comprising the polypeptide recovered from the host cell.
Dependencies are inferred from the claim text.