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US 8,557,537 B2Granted
Method for the production of overproducing Staphylococcus aureus strains
AssigneeSanofi Pasteur Limited
InventorsBachra Rokbi, Celine Lafont
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Abstract
The present invention relates to a method for the production of an overproducing Staphylococcus aureus strain comprising: (a) culturing a Staphylococcus aureus strain on a culture medium M1, M2 or M3, (b) optionally, recovering the strains thus produced from the culture medium, and also to the use of said strains for the production of polysaccharides.
Claims5
A method for the production of a Staphylococcus aureus serotype T5 or T8 capsular polysaccharide, comprising:
- (a) culturing a Staphylococcus aureus strain that produces a serotype T5 or T8 capsular polysaccharide on a culture medium comprising per liter of culture medium:
- (i) 52.5 to 200 g of plant peptone or 65 to 85 g yeast extract,
- (ii) 0 to 82.5 g of NaCl,
- (iii) 0 to 15 g of a magnesium salt selected from the group consisting of MgSO4 and MgCl2, and
- (iv) 0 to 7 g of sugar, the pH of which is within a range of values of from 6.3 to 6.7,
- (b) inactivating the culture thus produced, and
- (c) recovering the capsular polysaccharide.
4 more elements
Dependencies are inferred from the claim text.